TWILIGHTED is a groundbreaking Horizon Europe Twinning project that seeks to revolutionize deep-sea research, development and innovation (RD&I) in Portugal, ultimately benefiting all of Europe. Collaborating closely with the esteemed European institutions of GEOMAR (Germany) and NTNU (Norway), TWILIGHTED aims to transform ARDITI (Madeira, Portugal) into a global hub for deep-sea RD&I. Focusing on the challenging Mesophotic Zone (40-200m) and the enigmatic Twilight Zone (200-1,000m), and capitalizing on Madeira's unique proximity to deep waters, TWILIGHTED will help accelerate our global understanding of the ocean and its essential role in sustaining life on earth. TWILIGHTED’s key objectives are to: (1) Collaborate across research institutes in Europe, (2) Elevate the research profile of Portugal and especially the European Outermost Region of Madeira, (3) Innovate low-cost alternatives to state-of-the-art deep-sea research technologies, (4) Democratize deep-sea research, (5) Globalize deep-sea RD&I and (6) Share ocean science across stakeholders. To achieve its objectives, TWILIGHTED will adopt state-of-the-art approaches to training and networking. Development activities include staff exchanges, expert visits, training schools, joint research missions, the International Twilighted Conference and novel cross-sector workshops stimulating creativity in solving the ocean’s greatest challenges (the Impossible Things Workshops). Such capacity-building will not only catalyze deep-sea RD&I in Madeira, but ensure a lasting impact on the deep-sea scientific landscape, sustainable ocean policy and Madeira’s socioeconomic development. TWILIGHTED marks the start of a transformative journey for Portugal, redefining its role in understanding and protecting vital deep-sea ecosystems. TWILIGHTED will also facilitate greater diversity and collaboration in time-critical matters of ocean sustainability and leave a significant mark on the global pursuit for more democratic science.