The Project “Development of Human Biomonitoring Program for Latvia” (HBM4LV) plans to create knowledge for the evidence-based establishment of the human biomonitoring (HBM) program for Latvia benefiting from international experience and adapting it to the national context. This knowledge and evidence, as well as analysis of secondary data and available laboratory capacity, will be used to create guidelines for selecting a suitable cohort size for the general population and to elaborate on the most appropriate and feasible sampling strategy for biological samples. The Project will also ensure sampling of the selected cohort establishing Latvian HBM biobank and allow analysis of samples for most critical chemicals and their metabolites using national and, if, necessary international laboratories. The data of the Project will provide information on the most critical sources and exposure routes for pesticides, metals and persistent organic pollutants and their concentrations in biological samples of Latvian citizens which will serve as background for further recommendations for the development and implementation of the national HBM4LV program.