To create a sustainable chemical industry in Europe, we need to harness innovative breakthrough technologies. Industrial biotechnology is already making strides in chemical manufacturing by offering processes that are more efficient, specific, safer, and less energy-intensive. However, the industry faces a limitation: there are not enough types of enzymes being used. The W-BioCat project aims to expand the range of enzymes available, specifically by focusing on tungsten-containing enzymes (W-enzymes). W-enzymes have the ability to facilitate complex chemical reactions, particularly those that involve low redox potential reductions. Unfortunately, producing these enzymes on a large scale and at a reasonable cost has been a challenge. To overcome this, we plan to produce W-enzymes using an industrially friendly microorganism, E. coli. One big hurdle is the difficulty of synthesizing the tungsten cofactor for these enzymes. We're addressing this issue through advanced computational enzyme design techniques. Our goal is to create a completely new pathway for producing the tungsten cofactor in E. coli. With Hop-on W-BioCat, we will introduce a new W-enzyme expression system using the microorganism Aromatoleum evansii. This system has already proven effective in producing W-enzyme AaAOR from Aromatoleum aromaticum. Notably, AaAOR can oxidize H2, enabling it to catalyze the reduction of carboxylic acids to aldehydes or converting NAD+ to NADH. This innovation allows us to streamline the reaction, using just one enzyme instead of two to drive the process with H2. By incorporating AaAOR into our project, we gain new ways to achieve the objectives of the W-BioCat project, particularly demonstrating a hydrogen-driven conversion of oleic acid—derived from plants—into the emollient ester oleyl oleate. The expansion of the W-BioCat project with Hop-on W-BioCat offers significant synergies that will enhance our prospects to provide W-enzymes for a sustainable chemical industry.