The innovation ecosystem of the EU is suffering from a strong fragmentation with a big gap between innovation leader regions and emerging innovators. This divide severely limits the potential for innovative Start-ups from emerging and moderate innovator region to access capital and get adequate support for bringing their innovations to market. To tackle these challenges, INNOVATE-EU will strengthen and connect five local innovation ecosystems: the innovation leader region Baden-Württemberg (BW) in Germany, the moderate innovator regions of Lithuania and Aragon in Spain and the emerging innovator regions of Latvia and Northeast Romania. Its goal is to address the innovation gap between these regions, connect them to an EU-wide network of innovation ecosystems, and strengthen their deep tech Start-ups and Scale-ups by building on three (fundamental) pillars: i) a detailed analysis of the target groups and needs of the local innovation ecosystems followed by knowledge exchange and a detailed action plan to address the disparities, ii) the formation of the interconnected and inclusive INNOVATE-EU innovation support community and its connection to European communities, iii) direct support and trainings for promising deep tech Start-ups and Scale-ups providing them with the necessary knowledge, tools, and contacts for successfully upscaling their business models and bringing their products to market. Through this approach INNOVATE-EU will create an interconnected and inclusive innovation ecosystem with access to a wider European innovation support community and concrete pathways for existing and emerging deep tech entrepreneurs to access growth and investment opportunities. This will result in the overcoming of the innovation gap between moderately and highly innovative regions in Europe and the unveiling of disruptive and otherwise hidden Start-ups and Scale-ups in deep tech.