Biological surfactants are a family of natural molecules obtained from the microbial digestion of fatty acids and sugars. These compounds are developed the low impact of the synthesis process (industrial biotechnology), low toxicity and high biodegradability. Sophorolipids, glucolipids or cellobioselipids are some of the most important molecules. Historically developed as biodegradable detergents to replace petrochemicals, their high cost/benefit ratio stimulates their use for other high-end applications. Recent work demonstrates their self-assembly properties into fibers and bilayers, which show the formation of shear-thinning stimuli-responsive hydrogels. This class of soft materials is an interesting alternative to known matrices for tissue engineering, a field in continuous seek for new biomaterials due to problems of contamination, purity, cell adhesion and cost. The goal of SELFAMPHI is to use biocompatible amphiphiles to develop new injectable and printable hydrogels to test in tissue engineering (spinal disc repair and skin fillers) applications.