Protozoan and Metazoan parasites frequently infest edible fishes worldwide. Some of them are both fish pathogens and recognized agents of important zoonoses with high public health impact. In addition, uncertainty exists about the potential ability of other fish pathogens to infect humans. Meanwhile, parasitized fish can be found often enough in European fish stores. Furthermore, some parasites of fish are able to alter the organoleptic properties of fish products, having therefore a negative impact on fish industry. For these reasons, the present project is targeting fish parasites with impact on both health of consumers and quality of seafood. It proposes a reappraisal of fish-parasite related hazards by developing cost-effective innovative methods to detect eukaryotic pathogens in edible fish, associated with adapted training programs. It focuses mainly on both Anisakidae and Diphyllobothriidae larvae that cause two emerging helminthic diseases: anisakiasis and diphyllobothriasis, respectively. The growing consuming of marinated (or not) raw food, "exotic" or takeaway dishes and marked increase of out-of-home-food could favour the emergence of both typical anisakiasis and Anisakis-induced allergy. Interestingly, 43 % of restaurants and delicatessens did not freeze fish or did not use frozen fish for raw fish dishes. Likewise, the number of human cases of diphyllobothriasis due to the consumption of raw or undercooked freshwater fish from Alpine Lakes increased for the last years. This proposal targeted also the Apicomplexan protists of Cryptosporidium genus. In short, the pathogenic power to humans of fish-derived Cryptosporidium is unknown. We showed recently that C. molnari (from dorada and sea bass) was unable to infect SCID mice. We do not know, however, if other fish-derived Cryptosporidium species or strains are infectious to mammals. The diversity of Cryptosporidium genus could be important in sea- or freshwater teleosteens. We are able to detect, identify and describe new Cryptosporidium species on molecular and phenotypic bases as well as to explore their pathogenic power to mammals in a highly reproducible experimental model well mastered by BDEEP members. The present project is consistent with the "experimental development" kind of research ('Call for proposals', p 24), the integrative online approach proposed here search for "acquiring, associating and using knowledge and scientific, technological, and training methods" in order to generate updated conceptions about edible-fish parasites, and innovative procedures to detect and identify them. Moreover, the present project is suitable for thematic axis 2 of the call for proposals ("Research in alimentation for a more dynamic market of food production") for the following reasons: (a) it proposes a rationale screening activity of parasites harboured by edible fish as an added technological value integrated into an industrial structure or business establishment; (b) it aims at integrating health safety, nutritional quality, organoleptic quality, practical wisdom and reasonable costs; (c) it proposes the combination of know-how of IFREMER specialists in quality of fish products, AFSSA specialists in microbiological health risk and protection of consumers, CEVPM specialists in RD and biotechnology of seafood products, skill of LASMEA and ARBOR Technology in conception and manufacturing of device for food industry; (d) parasitology specialists of three recognized centres: 'Biology & Diversity of Emerging Eukaryotic Pathogens' service of Lille Pasteur Institute (BDEEP, EA3609-Lille-2 University, formerly 'Ecology of Parasitism'), specialized in emerging parasitic Protists and protist-like organisms, Parasitology-Mycology Central Lab of Cochin Hospital (Paris), specialized in Diphyllobothridae tapeworms and Parasitology laboratory of Public Health Sciences, DSSP, Sapienza Roma University (Rome) specialized in metazoan parasites of fish and marine mammals.