Facing the need to reduce the use of petroleum resources, limit greenhouse gas emissions and recover waste, lignocellulosic biorefineries appear as a promising solution for the production of platform molecules. In this context, the GLUXY project aims to develop an innovative process scheme combining enzymatic hydrolysis, fermentation and membrane separation steps for the production of 2 platform molecules: glutamic acid and xylitol identified among the economically interesting molecules. The originality lies in the co-fermentation of the two types of sugar resulting from the hydrolysis of biomass, pentoses (C5) and hexoses (C6), without prior separation, by a mixed culture of two microorganisms, a bacteria that produces glutamic acid from C6 and a yeast that produces xylitol from C5. Several types of grain bran will be used as lignocellulosic feedstocks to prepare C6 and C5 hydrolysates. The separation steps will involve membrane techniques, nanofiltration and electrodialysis. The challenge is to limit the number of process steps, and therefore the cost and environmental impact of the produced molecules. The assessment of the environmental impacts of this process scheme will be carried out via a Life Cycle Assessment (LCA). One of the strengths of this project is the simultaneous study of the entire transformation chain that will take into account the complexity and variability of raw materials but also the interactions between each of these steps.