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This proposal is foccused at developing a Low Molecular Weight Gel (LMWG) technology to serve as a smart scaffold for stem cell culture and in situ delivery systems. GELCELLS is based on the complementary chemistry/biology expertises of two partners. The methodology utilized in the proposed studies will marry the expertise of organic chemistry, physico-chemistry, cellular biology, stem cell cultures and a range of techniques to accomplish a comprehensive description of the systems developed. To achieve this ambitious goal, the following tasks have been defined and are listed below. All the tasks can be achieved within the time frame of this project (see figure and table below for the link between the different workpackages of this program). 1. Coordination (INSERM U869, U. Bordeaux) 2. Synthesis of GNLs (INSERM U869, U. Bordeaux) 3. Physico-chemical studies (INSERM U869, U. Bordeaux). Identification of the key physical and structural characteristics of the LMWGs 4. Cells/hydrogel interaction studies in 2D and 3D systems (INSERM U577) 5. Study of nucleic acid entrapment and release (INSERM U869, U. Bordeaux) 6. Transfection assays with matricial systems (INSERM U577, INSERM U869, U. Bordeaux) The following results will be used for patents and/or publications: • Library of glycosylated GNLs • A control of the molecular, supramolecular and physicochemical properties of the LMWG gel matrix and their influence on the cell fate • Successful stem cell culture in LMWG systems • Controlled entrapment and delivery of nucleic acids by the LMWGs matrices • Successful in situ transfection in 2D and 3D matrix
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