Innovations in food packaging mostly concern food shelf-life and consumer safety by the inhibition or prevention of microbial growth onto food, thanks to the development of antimicrobial active packaging. In particular, bio-based biodegradable polymers and antimicrobial natural compounds generate a growing interest in the sustainability of packaged food. The aim of NanoBAP project is to investigate the potential of electrospun nanofibers in the field of active packaging, through the development of an antimicrobial and antioxidant coating based on biosourced materials for the combined release of multiple natural active compounds from a PLA film. Two strategies based on electrospinning will be fully investigated: from the design and characterisation of physico-chemical properties of the coated films and the release/transfer mechanisms of active compounds up to the evaluation of in vitro and model/simplified food antimicrobial activity. The final innovative proposed packaging solution would be of key importance for the packing of sliced or textured fresh foods. In conclusion, the outcome of this project will generate fully bio-based and biodegradable active films with the potential to substantially mitigate plastic pollution and to reduce food waste. This will make a both scientific and economical step forward to “zero waste” concept.