Complex microbial communities underlie natural processes such as global chemical cycles and digestion in higher animals, and are routinely exploited for industrial scale synthesis, waste treatment and fermentation. Our basic understanding of the structures, stabilities and functions of such communities is limited, leading to the declaration of their study as the next frontier in microbial ecology, microbiology, and synthetic biology. Focusing on biomethane producing microbial communities (BMCs), we will undertake a two-tiered approach of optimising natural communities and designing synthetic communities with a focus on achieving robust, high-yield biomethane production. Within this biotechnological framework, our proposal will address several fundamental scientific questions on the link between the structure and function of microbial communities. To ensure success in this challenging project, we assembled the strongest possible interdisciplinary research team that combines significant practical and scientific expertise in microbial ecology and evolution, systems modelling, molecular microbiology, bioengineering, genomics, and synthetic biology. We are confident that this team will deliver and that this project will result in significant impact in the scientific and industrial domains. Through our work, described in detail below, we will; significantly improve the current understanding of the structure-function relation in microbial communities, provide the scientific community with a systematic, temporal genomics and transcriptomics dataset on complex microbial communities, develop novel computational tools for microbial community (re)design, and experimentally build synthetic BMCs that will act as model ecosystems in different research fields. These scientific developments, in turn, will accumulate in the development of more sustainable bioenergy solutions for the UK economy by optimising the communities underlying biomethane production. This will help to drive the efficiency of biomethane as an alternative fuel source.

In a circular economy, clean growth is achieved by increasing the value derived from existing and planned economic infrastructure, products and materials which in turn significantly reduces or eliminates negative externalities. Increased value can be achieved by maintaining the integrity of a product or material at a higher level, using products longer, cascading their use in adjacent value chains and designing pure, high quality feedstocks (avoiding contamination and toxicity). A circular economy approach to plastics addresses simultaneously the accumulation, impact and costs in the environment whilst maintaining applications for multiple high value purposes. To translate potential to reality requires new circular plastics systems that a) co-ordinate and integrate key system players and activities across the value chain b) are underpinned by rigorous scientific research evidence; c) promote novel and creative approaches to the circulation and cascading of plastics in society and; d) demonstrate and proof points in support of decision-making and action at varying s Ths proposal will connect excellent institutional research activities within a single highly visible Multidisciplinary Plastics Research Hub - "ExeMPlaR" led by the University of Exeter to provide the first stage in a comprehensive, systematic and coordinated approach to the formation of a novel and creative circular economies, using regional demonstrators in the SW of England to test a number of key building blocks. This will be based on system-oriented innovation and high quality inter-disciplinary and collaborative scientific research within a proven, cohesive circular economy framework to address both the cause(s) of the problems and efforts to solve them rather than just treating the symptoms. This research effort involves the demonstration of the technical feasibility and superior economic, material, health, environmental and social value of a circular economy system re-design against a current linear base case. Expert-led, technical solutions by themselves however are unlikely to be effective and require in addition a theory of change that connects human behaviours, social systems and structures with circular economy principles. ExeMPLaR will bring together business, policy, community, environmental, and media representatives with a shared 'narrative' (in this case , a new Circular Plastics Economy) values and ideas, to jointly identify and work on a complex set of activities and pilot projects, that together form an effective innovation ecosystem (WP1). EXeMPLaR will undertake a novel and creative approach to impact by applying the principles of networks of transformative change into a circular economy project. ExeMPLaR therefore focusses on the current plastic system and address the potential to create higher value from existing plastic flows, create new opportunities for regional design and closed loop manufacturing and community initiatives, reduce negative externalities and create networks for transformational change to co-design and support systems innovations required at regional scale. To achieve this vision many challenges have to be overcome. To start the process of creating effective regional plastics economies, ExeMPLaR will synthesise an authoritative evidence base to inform regional actions, interventions and evaluation. This will build on a wide range of world leading plastics research at Exeter. We will translate these findings into the first stage of an evaluation tool and apply these to three front runner regional interventions, and additional smaller projects co-designed and prioritized by our network, to test opportunities for re-using, replacing or eliminating certain categories of fossil fuel derived plastic. After testing the impacts, outcomes and value creation potential we will address the potential challenges and enablers to replication and scaling these interventions at regional and national scale.

Complex microbial communities underlie natural processes such as global chemical cycles and digestion in higher animals, and are routinely exploited for industrial scale synthesis, waste treatment and fermentation. Our basic understanding of the structures, stabilities and functions of such communities is limited, leading to the declaration of their study as the next frontier in microbial ecology, microbiology, and synthetic biology. Focusing on biomethane producing microbial communities (BMCs), we will undertake a two-tiered approach of optimising natural communities and designing synthetic communities with a focus on achieving robust, high-yield biomethane production. Within this biotechnological framework, our proposal will address several fundamental scientific questions on the link between the structure and function of microbial communities. To ensure success in this challenging project, we assembled the strongest possible interdisciplinary research team that combines significant practical and scientific expertise in microbial ecology and evolution, systems modelling, molecular microbiology, bioengineering, genomics, and synthetic biology. We are confident that this team will deliver and that this project will result in significant impact in the scientific and industrial domains. Through our work, described in detail below, we will; significantly improve the current understanding of the structure-function relation in microbial communities, provide the scientific community with a systematic, temporal genomics and transcriptomics dataset on complex microbial communities, develop novel computational tools for microbial community (re)design, and experimentally build synthetic BMCs that will act as model ecosystems in different research fields. These scientific developments, in turn, will accumulate in the development of more sustainable bioenergy solutions for the UK economy by optimising the communities underlying biomethane production. This will help to drive the efficiency of biomethane as an alternative fuel source.

FIWARE is a smart solution platform, funded by the EC (2011-16) as a major flagship PPP, to support SMEs and developers in creating the next generation of internet services, as the main ecosystem for Smart City initiatives for cross-domain data exchange/cooperation and for the NGI initiative. So far little progress has been made on developing specific water-related applications using FIWARE, due to fragmentation of the water sector, restrained by licensed platforms and lagging behind other sectors (e.g. telecommunications) regarding interoperability, standardisation, cross-domain cooperation and data exchange. Fiware4Water intends to link the water sector to FIWARE by demonstrating its capabilities and the potential of its interoperable and standardised interfaces for both water sector end-users (cities, water utilities, water authorities, citizens and consumers), and solution providers (private utilities, SMEs, developers). Specifically we will demonstrate it is non-intrusive and integrates well with legacy systems. In addition to building modular applications using FIWARE and open API architecture for the real time management of water systems, Fiware4Water also builds upon distributed intelligence and low level analytics (smart meters, advanced water quality sensors) to increase the economic (improved performance) and societal (interaction with the users, con-consensus) efficiency of water systems and social acceptability of digital water, by adopting a 2-Tier approach: (a) building and demonstrating four Demo Cases as complementary and exemplary paradigms across the water value chain (Tier#1); (b) promoting an EU and global network of followers, for digital water and FIWARE (cities, municipalities, water authorities, citizens, SMEs, developers) with three complementary Demo Networks (Tier#2). The scope is to create the Fiware4Water ecosystem, demonstrating its technical, social and business innovative potential at a global level, boosting innovation for water.

Land-use and agriculture are responsible for around one quarter of all human greenhouse gas (GHG) emissions. While some of the activities that contribute to these emissions, such as deforestation, are readily observable, others are not. It is now recognised that freshwater ecosystems are active components of the global carbon cycle; rivers and lakes process the organic matter and nutrients they receive from their catchments, emit carbon dioxide (CO2) and methane to the atmosphere, sequester CO2 through aquatic primary production, and bury carbon in their sediments. Human activities such as nutrient and organic matter pollution from agriculture and urban wastewater, modification of drainage networks, and the widespread creation of new water bodies, from farm ponds to hydro-electric and water supply reservoirs, have greatly modified natural aquatic biogeochemical processes. In some inland waters, this has led to large GHG emissions to the atmosphere. However these emissions are highly variable in time and space, occur via a range of pathways, and are consequently exceptionally hard to measure on the temporal and spatial scales required. Advances in technology, including high-frequency monitoring systems, autonomous boat-mounted sensors and novel, low-cost automated systems that can be operated remotely across multiple locations, now offer the potential to capture these important but poorly understood emissions. In the GHG-Aqua project we will establish an integrated, UK-wide system for measuring aquatic GHG emissions, combining a core of highly instrumented 'Sentinel' sites with a distributed, community-run network of low-cost sensor systems deployed across UK inland waters to measure emissions from rivers, lakes, ponds, canals and reservoirs across gradients of human disturbance. A mobile instrument suite will enable detailed campaign-based assessment of vertical and spatial variations in fluxes and underlying processes. This globally unique and highly integrated measurement system will transform our capability to quantify aquatic GHG emissions from inland waters. With the support of a large community of researchers it will help to make the UK a world-leader in the field, and will facilitate future national and international scientific research to understand the role of natural and constructed waterbodies as active zones of carbon cycling, and sources and sinks for GHGs. We will work with government to include these fluxes in the UK's national emissions inventory; with the water industry to support their operational climate change mitigation targets; and with charities, agencies and others engaged in protecting and restoring freshwater environments to ensure that the climate change mitigation benefits of their activities can be captured, reported and sustained through effectively targeted investment.