GoodByO seeks at creating a new generation multi-commodities biorefinery based on food waste, biogenic CO2, and bioprocess wastewaters for resource-efficient valorization of sustainable feedstocks. GoodByO will apply this visionary concept at ChainCraft BV bio-plant by valorizing its gaseous and liquid side-streams as zero-cost feedstocks. By exploiting the metabolic diversity of microbial catalysts, four different microbial factories (MF) will be developed: -MF1: open culture chain elongation process for bio-octanoic acid production; -MF2: gas fermentation process for bio-hexanol production from biogas and green H2; -MF3: novel anaerobic photoautotrophic biogas desulfurization process; - MF4: wastewaters-based mixotrophic microalgal process for high-value carotenoids production. The project target relies in the validation of long-term production stability of the developed bioprocesses at TRL5, using real feedstocks, to provide transferable outcomes for further scale-up at higher TRLs. GoodByO will also design an in silico 100% renewable energy system capable of sustaining the fluctuating demand of the integrated biorefinery over time, by adopting biomethanation process as grid-balancing system. GoodByO targets to produce biobased products meeting market requirements at cost-competitive selling price with benchmarks, to boost end users companies in substituting fossil- and palm-oil based products with biobased ones. The successful scale-up and the market penetration of GoodByO processes will strongly increase EU global leadership in the Manufacturing biotech-Industry while advancing CO2-utilizing biotechnologies for developing a carbon-negative economy.

CELBICON aims at the development, from TRL3 to TRL5, of new CO2-to-chemicals technologies, conjugating at once small-scale for an effective decentralized market penetration, high efficiency/yield, low cost, robustness, moderate operating temperatures and low maintenance costs. In line with the reference Topic text, these technologies will bridge cost-effective CO2 capture and purification from the atmosphere through sorbents (with efficient heat integration of the CO2 desorption step with the subsequent process stages), with electrochemical conversion of CO2 (via PEM electrolysis concepts, promoting CO2 reduction at their cathode in combination with a fruitful oxidation carried out simultaneously at the anode), followed by bioreactors carrying out the fermentation of the CO2-reduction intermediates (syngas, C1 water-soluble molecules) to form valuable products (bioplastics like Poly-Hydroxy-Alkanoates - PHA -, isoprene, lactic acid, methane, etc.) as well as effective routes for their recovery from the process outlet streams. A distinctive feature of the CELBICON approach is the innovative interplay and advances of key technologies brought in by partners (high-tech SMEs & companies, research centres) to achieve unprecedented yield and efficiency results along the following two processing lines: i) High pressure process line tailored to the production of a PHA bioplastic and pressurized methane via intermediate electrochemical generation of pressurized syngas followed by specific fermentation steps; ii) Low pressure processing line focused on the production of value-added chemicals by fermentation of CO2-reduction water-soluble C1 intermediates. Over a 42 months project duration, the two process lines described will undergo a thorough component development R&D programme so as to be able to assemble three optimised TRL5 integrated test-rigs (one per TP) to prove the achievement of all the quantified techno-economical targets.

The ENGICOIN proposal aims at the development, from TRL3 to TRL5, of three new microbial factories (MFs), integrated in an organic waste anaerobic digestion (AD) platform, based on engineered strains exploiting CO2 sources and renewable solar radiation or H2 for the production of value-added chemicals, namely: MF.1) the cyanobacteria Synechocystis to produce lactic acid from either biogas combustion flue gases (CO2 concentration ~ 15%) or pure and costless CO2 streams from biogas-to-biomethane purification. MF.2) the aerobic and toxic metal tolerant Ralstonia eutropha to produce PHA bioplastics from biogas combustion flue gases and complementary carbon sources derived from the AD digestate. MF.3) the anaerobic Acetobacterium woodii to produce acetone from the CO2 stream from biogas-to-biomethane purification. High process integration will be guaranteed by taking advantage of low-grade heat sources (e.g. from cogenerative biogas-fired engine or an tailored PEM electrolyser), exploitable side gas streams (e.g. O2 from electrolysis, CO2 from biomethane purification), low-price electricity produced during night-time by a biogas-fired-engine cogeneration unit or even intensified operation conditions (e.g. up to 10 bars pressure for the anaerobic acetone production bioreactor; led-integrated photo-bioreactor). This is an essential feature, alongside with the high conversion rates enabled by synthetic and systems biology on the above microorganisms, to achieve competitive selling prices for the key target products (1.45 €/kg for lactic acid; 3.5 €/kg for PHA; 1 €/kg for acetone). Notwithstading the key application platform (anaerobic biorefinery based on organic wastes) the innovative production processes developed have a great exploitation potential in other application contexts: flue gases from different combustion appliances (e.g. cement kilns), alcoholic fermentation CO2 streams (e.g. lignocelluosic biorefineries, breweries), etc.