Anaerobic digestion (AD) is a technology where microorganisms break down organic matter to produce biogas, thereby generating renewable energy from waste. Biogas can be combusted to produce electricity or purified and used as a substitute for natural gas (NG). Because it provides a carbon-neutral substitute for fossil fuels, while also preventing methane emissions at landfills by processing organic waste, AD is noted as an important part of the UK Net Zero Strategy: Build Back Greener. This project aims to develop artificial intelligence (AI) tools to enable radical efficiency improvements in AD biogas production. Currently, there are about 650 operational AD sites in the UK, which reduce UK greenhouse gas emissions by an estimated 1%. This contribution is meaningful, but modest in comparison to AD's potential. The fundamental roadblock at present is a lack of flexibility. Due to the complexities of predicting how different waste feedstocks and different microbial communities will interact under varying operating conditions, AD biogas producers must minimise risk by purchasing only the highest-quality, consistent feedstock, which may also be seasonal; any errors could result in long and costly downtimes. Thus, available waste streams are vastly under-utilised; feedstock prices are driven up, weakening the economic viability of AD biogas production; and limited feedstocks may need to be transported longer distances, increasing carbon emissions. AI holds crucial promise for the optimisation and future expansion of AD biogas production. As an industry that does not have the central research capabilities of other large energy sectors, it furthermore presents exceptional challenges due to the complexities and inherent uncertainties across interacting chemical, biological, and - if reductions in total life-cycle emissions are to be achieved - environmental systems. The project team therefore unites expertise in AI, process optimisation, systems microbiology, and life-cycle assessment to develop whole-systems decision-making tools informed by detailed sub-system modelling. The outputs will include decision-making tools, specifically: A) a hybrid machine-learning digital twin of the biodigesters, based on novel mechanistic modelling approaches combined with process data from industrial partners and new experimental data from the project; and B) optimisation-based system models of other components of a site, to perform site-wide real-time optimisation through a multi-layer digital twin that includes economic and environmental indicators. By linking the digital twin of the biodigester to feedstock procurement and downstream processes, it will be possible to quickly determine the impact of different feedstocks, their combinations, and their prices on biogas quality, while also tracking quantified environmental impacts across AD value chains in real-time and assessing negative emissions potential in future. Increasing the flexibility of UK AD industry will expand waste markets and lower prices to grow the sector with more capacity, boost profits and productivity, and enhance the overall attractiveness of AD as an investment. Increasing biogas output will help lower UK dependence on foreign NG sources and lower overall emissions from the energy system. The project is supported by partners from across the UK to ensure the aims and objectives can be met, to result in a step-change in the AD industry and position the UK as a global AD leader. The knowledge, tools, and methods developed will be applicable in wastewater treatment, where AD is also used. Beyond that, our AI approaches to systems biology will have potential for widespread application in bioprocessing sectors more generally, such as biopharmaceuticals, biofuels, food, and fermentation. With our network of partners, we will explore potential commercialisation and licencing of our digital techniques to maximise impact and work across sectors toward the common goal of Net Zero.

Terrestrial farming is the greatest driver of biodiversity loss, a major contributor to greenhouse gas emissions and water pollution, and faces its most transformational reform in 50 years to improve both environmental and economic sustainability. The new Agriculture Act, 25YEP, has commitment to net zero carbon emissions and policies to enhance environmental stewardship, sustainability and support the production of public goods. This project aims to demonstrate the socio-economic benefit of a world-leading 'terrestrial blue economy', contributing multiple public goods to reform UK agriculture. Combining high value shrimp aquaculture with farm-based renewable energy will provide a novel home-grown output with considerable but poorly understood economic and health potential. The public goods benefits of a switch from beef/sheep production to shrimp include lower greenhouse gas emissions, water pollution, and land use, freeing land for other public goods such as trees, biodiversity, biodiversity net gain, and recreation. Furthermore, co-locating self-contained, indoor shrimp production units with UK farm anaerobic digesters (AD) will maximise use of their (otherwise wasted) heat energy, enhancing sustainability and circularity of both industries. Extra income will also boost the farm-based renewable energy sector, helping the UK meet emissions targets. Shrimp is a healthy seafood with high protein, low calories, low fat, rich in vitamins, minerals and antioxidants, promoting brain and heart health. Warm water shrimp is already highly popular seafood in the UK, with 22,852 tons (UK retail £319M) imported annually from Central America and SE Asia. However, traditional overseas production is vulnerable to climate/disease crises, has high transport-related CO2 emissions, and often uses environmentally unsustainable practices, e.g., destroying up to 80 % of nations' mangrove forests which absorb and trap more CO2 than any other of Earth's ecosystems. They also provide coastal protection against storms and coastal erosion. There is also the problem of illegal use (or just misuse) of chemicals such as pesticides and antibiotics resulting in contaminant residues in some of the shrimp exported to the UK, EU and US that can cause health issues. This proposal aims to completely avoid these problems and ensure a risk-free, healthier and sustainable supply chain of this heart- and brain- healthy seafood for UK-consumers, by facilitating a major expansion of UK's shrimp RAS production sector which currently supplies equivalent to <1% of imports. We aim to co-locate RAS production with renewable energy sources on UK terrestrial farms. We conservatively estimate that if only 20% of the UK's current Anaerobic Digestor (AD) plants were adapted for shrimp farming, we could sustain 960 shrimp production units and harvest 5,520 tonnes of shrimp per year (~25 % of current UK warm water shrimp imports). With the rapid growth of AD plants across UK farms (10-fold increase since 2010), there is clear potential for truly sustainable, healthier, home-grown shrimp to provide the majority consumed in the near future, in addition to enhancing environmental stewardship, sustainability and supporting the production of public goods from UK agricultural practices. Importantly, this project will generate data to evaluate the true potential of sustainable UK shrimp production using renewable energy technology, as well as providing this shrimp industry with the necessary world-class scientific support. This project will therefore address 3 goals to transform the UK Food System: 1) increased environmental sustainability of farm practices (e.g., sustainable use of existing waste heat from ADs), 2) economically sustainable expansion of UK land-based aquaculture production & employment, and 3) establishing the UK as a leader regarding capability, expertise and innovation in co-reforming agriculture and aquaculture.