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A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini

Authors: Tim Kamber; Nachelli Malpica-López; Monika Messmer; Thomas Oberhänsli; Christine Arncken; Joris Alkemade; Pierre Hohmann;

A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini

Abstract

White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes severe yield losses. The aim of this work was to develop a C. lupini-specific quantitative real-time TaqMan PCR assay that allows for quick and reliable detection and quantification of the pathogen in infected seed and plant material. Quantification of C. lupini DNA in dry seeds allowed us to distinguish infected and certified (non-infected) seed batches with DNA loads corresponding to the disease score index and yield of the mother plants. Additionally, C. lupini DNA could be detected in infected lupin shoots and close to the infection site, thereby allowing us to study the disease cycle of this hemibiotrophic pathogen. This qPCR assay provides a useful diagnostic tool to determine anthracnose infection levels of white lupin seeds and will facilitate the use of seed health assessments as a strategy to reduce the primary infection source and spread of this disease.

Country
Spain
Subjects by Vocabulary

Microsoft Academic Graph classification: Veterinary medicine Crop Lupinus Colletotrichum lupini TaqMan Cultivar Pathogen Legume biology biology.organism_classification Shoot

Keywords

<i>Lupinus albus</i>, Crop health, quality, protection, 632, Plant Science, <i>Colletotrichum</i> <i>acutatum</i> species complex, Article, hydrolysis probe, anthracnose; Colletotrichum acutatum species complex; Colletotrichum lupini; hydrolysis probe; lifecycle; Lupinus albus; qPCR; seed, Colletotrichum acutatum species complex, Ecology, Evolution, Behavior and Systematics, Colletotrichum lupini, <i>Colletotrichum</i> <i>lupini</i>, anthracnose, Ecology, Botany, Breeding, genetics and propagation, lifecycle, qPCR, Lupinus albus, QK1-989, seed

28 references, page 1 of 3

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3. Schulze, J.; Temple, G.; Temple, S.J.; Beschow, H.; Vance, C.P. Nitrogen fixation by white lupin under phosphorus deficiency. Ann. Bot. 2006, 98, 731-740. [CrossRef]

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6. Nirenberg, H.I.; Feiler, U.; Hagedorn, G. Description of Colletotrichum lupini comb. nov in modern terms. Mycologia 2002, 94, 307-320. [CrossRef]

7. Damm, U.; Cannon, P.F.; Woudenberg, J.H.; Crous, P.W. The Colletotrichum acutatum species complex. Stud. Mycol. 2012, 73, 37-113. [CrossRef] [PubMed] [OpenAIRE]

8. Talhinhas, P.; Baroncelli, R.; Le Floch, G. Anthracnose of lupins caused by Colletotrichum lupini: A recent disease and a successful worldwide pathogen. J. Plant Pathol. 2016, 98, 5-14. [OpenAIRE]

9. Alkemade, J.A.; Messmer, M.M.; Voegele, R.T.; Finckh, M.R.; Hohmann, P. Genetic diversity of Colletotrichum lupini and its virulence on white and Andean lupin. Sci. Rep. 2021, 11, 13547. [CrossRef] [PubMed] [OpenAIRE]

10. White, P.; French, R.; McLarty, A. Producing Lupins; Department of Agriculture and Food: Perth, Australia, 2008.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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