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A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini
White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes severe yield losses. The aim of this work was to develop a C. lupini-specific quantitative real-time TaqMan PCR assay that allows for quick and reliable detection and quantification of the pathogen in infected seed and plant material. Quantification of C. lupini DNA in dry seeds allowed us to distinguish infected and certified (non-infected) seed batches with DNA loads corresponding to the disease score index and yield of the mother plants. Additionally, C. lupini DNA could be detected in infected lupin shoots and close to the infection site, thereby allowing us to study the disease cycle of this hemibiotrophic pathogen. This qPCR assay provides a useful diagnostic tool to determine anthracnose infection levels of white lupin seeds and will facilitate the use of seed health assessments as a strategy to reduce the primary infection source and spread of this disease.
- University of Basel Switzerland
- Research Institute of Organic Agriculture Belgium
- Institut de Recerca i Tecnologia Agroalimentàries Spain
Microsoft Academic Graph classification: Veterinary medicine Crop Lupinus Colletotrichum lupini TaqMan Cultivar Pathogen Legume biology biology.organism_classification Shoot
<i>Lupinus albus</i>, Crop health, quality, protection, 632, Plant Science, <i>Colletotrichum</i> <i>acutatum</i> species complex, Article, hydrolysis probe, anthracnose; Colletotrichum acutatum species complex; Colletotrichum lupini; hydrolysis probe; lifecycle; Lupinus albus; qPCR; seed, Colletotrichum acutatum species complex, Ecology, Evolution, Behavior and Systematics, Colletotrichum lupini, <i>Colletotrichum</i> <i>lupini</i>, anthracnose, Ecology, Botany, Breeding, genetics and propagation, lifecycle, qPCR, Lupinus albus, QK1-989, seed
<i>Lupinus albus</i>, Crop health, quality, protection, 632, Plant Science, <i>Colletotrichum</i> <i>acutatum</i> species complex, Article, hydrolysis probe, anthracnose; Colletotrichum acutatum species complex; Colletotrichum lupini; hydrolysis probe; lifecycle; Lupinus albus; qPCR; seed, Colletotrichum acutatum species complex, Ecology, Evolution, Behavior and Systematics, Colletotrichum lupini, <i>Colletotrichum</i> <i>lupini</i>, anthracnose, Ecology, Botany, Breeding, genetics and propagation, lifecycle, qPCR, Lupinus albus, QK1-989, seed
Microsoft Academic Graph classification: Veterinary medicine Crop Lupinus Colletotrichum lupini TaqMan Cultivar Pathogen Legume biology biology.organism_classification Shoot
28 references, page 1 of 3
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8. Talhinhas, P.; Baroncelli, R.; Le Floch, G. Anthracnose of lupins caused by Colletotrichum lupini: A recent disease and a successful worldwide pathogen. J. Plant Pathol. 2016, 98, 5-14. [OpenAIRE]
9. Alkemade, J.A.; Messmer, M.M.; Voegele, R.T.; Finckh, M.R.; Hohmann, P. Genetic diversity of Colletotrichum lupini and its virulence on white and Andean lupin. Sci. Rep. 2021, 11, 13547. [CrossRef] [PubMed] [OpenAIRE]
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