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Article . 2023
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Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods

Authors: Smith P.; Le Devendec L.; Jouy E.; Larvor E.; Le Breton A.; Picon-Camacho S.; Zrnčić S.; +23 Authors

Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods

Abstract

This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested applying standard methods specifying incubation at 28 °C for 24-28h. Aggregated MIC distributions for a total of 247 isolates were determined in nine laboratories for eleven agents. Data aggregations of the disc zone for the ten agents analysed contained between 157 and 218 observations made by four to seven laboratories. Acceptable ranges for quality control (QC) reference strains were available for seven agents and the related multi-laboratory aggregated data were censored excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg/ml), gentamicin (≤4 µg/ml), oxytetracycline (≤0.25 µg/ml) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg/ml). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available or not, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole) and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to CLSI for their consideration in setting international consensus epidemiological cut-off values.

International audience

Countries
Denmark, France, Norway, Italy, Netherlands
Keywords

Antimicrobial suceptibility, Enrofloxacin, antimicrobial activity, Sulfamethoxazole, Oxolinic Acid, detection method, [SDV]Life Sciences [q-bio], Normalized resistance interpretation, Antimicrobial susceptibility; Aquatic animals; CLSI VET04; ECOFFinder; Normalized resistance interpretation, aquatic organism, Aquatic animals, Microbial Sensitivity Tests, ECOFFinder, Aquatic Science, Antimicrobial susceptibility, Trimethoprim, CLSI VET04, Animals, Life Science, epidemiology, Gentamicins, oxytetracycline, quality control, Ecology, Evolution, Behavior and Systematics

38 references, page 1 of 4

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Baron S, Smith P, Verner-Jeffreys DW (2020b) Setting epidemiological cut-off values for bacteria isolated from aquatic animals: a toolbox for designing a 96-well plate for microdilution MIC assays. Bull Eur Assoc Fish Pathol 40:55−61

CLSI (2017) Performance standards for antimicrobial susceptibility testing. 27th edn. CLSI document M100−S27. Clinical and Laboratory Standards Institute, Wayne, PA

CLSI (2018) Development of in vitro susceptibility testing criteria and quality control parameters. CLSI guideline M23, 5th edn. Clinical Laboratory Standards Institute Wayne, PA

CLSI (2020a) Methods for antimicrobial broth dilution and disk diffusion susceptibility testing of bacteria isolated from aquatic animals. CLSI guideline VET03, 2nd edn. Clinical Laboratory Standards Institute, Wayne, PA

CLSI (2020b) Performance standards for antimicrobial susceptibility testing of bacteria isolated from aquatic animals. CLSI guideline VET04, 3rd edn. Clinical Laboratory Standards Institute, Wayne, PA

EUCAST (2018) EUCAST system for antimicrobial abbreviations. https://www.eucast.org/fileadmin/src/media/PDFs/ EUCAST_files/Disk_test_documents/Disk_abbreviations/ EUCAST_system_for_antimicrobial_abbreviations.pdf

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    1
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
hybrid
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