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    Authors: Palagano R.; Valli E.; Tura M.; Cevoli C.; +4 Authors

    INTRODUZIONE Come ben noto, il contenuto in etil esteri degli acidi grassi (EEAGs) è considerato un parametro diretto di qualità da applicarsi per definire l’appartenenza di oli vergini di oliva (VOOs) ad una specifica categoria (limite fissato a ≤35 mg/kg per la categoria extra vergine di oliva per la legislazione UE) ed è anche ritenuto un parametro indiretto utile per mettere in luce possibili miscele fraudolente tra un olio extra vergine di oliva ed un olio soft deodorato [1]. In questo lavoro è stato proposto un approccio analitico alternativo al metodo ufficiale con lo scopo di offrire una procedura non in linea HPLC-GC-FID migliora tiva in termini di tempo di analisi, volume di solventi e livello di complessità. Più nello specifico, è stato impiegato l’HPLC per collezionare i EEAGs dagli oli vergini di oliva (VOOs) al posto della tradizionale cromatografia liquida su colonna. Inoltre, è stato testato anche un iniettore di vaporizzazione a temperatura programmata (PTV) come possibile alternativa a quello on-column poichè il PTV è considerato più versatile consentendo il controllo programmato e l’ottimizzazione della modalità di iniezione (split/splitless). Le prestazioni del metodo alternativo sono state valutate controllando i risultati ottenuti per differenti parametri che caratterizzano il protocollo di validazione interna [2]. A tale scopo, sono state analizzate tipologie specifiche di campioni quali olio di oliva raffinato aggiunto di cinque concentrazioni diverse di standard puri degli analiti di interesse e campioni reali VOOs caratterizzati da concentrazioni diverse di EEAGs (basso, medio ed alto contenuto di EEAGs) ed applicando in parallelo i due metodi (alternativo vs ufficiale). INTRODUCTION As well known, the content in fatty acid ethyl esters (FAEEs) represents a direct quality parameter to define the quality grade of virgin olive oil (VOOs) (limit set at ≤35 mg/kg oil for extra virgin olive oil category for EU regulation), and it is also considered an indirect parameter to detect fraudulent mixtures of EVOO with lower quality oils such as soft deodorized olive oils [1]. In this work, the official method has been revised to propose an alternative off-line procedure based on HPLC-GC-FID, more suitable in terms of time of analysis, volume of solvents and level of complexity. Specifically, the analytical protocol proposed HPLC for collecting FAEEs from VOOs instead of the traditional liquid chromatography of the official method. Moreover, a programmed temperature vaporizer (PTV) injector was tested as an alternative to the on-column one because PTV is considered a more versatile option as the temperature control is time-programmed and the injection mode (split/splitless) can be optimized. The performance of the alternative method was evaluated, checking the results in terms of several parameters regarding in-house validation protocol [2]. For this control, specific kinds of samples as refined olive oil spiked with five concentrations of a pure standard of analytes of interest and real VOOs characterized by different contents of FAEEs (low, medium, high) were analyzed applying in parallel the two methods (alternative vs official) (see the Figure). RESULTS The main advantages of this revised protocol may be summarized as: i) significant reduction in time and solvents needed (reduction of more than 50% and 80% for time and solvents, respectively compared to the official procedure); ii) simplification of the separation step of FAEEs fraction avoiding the manual packing of the glass column for the liquid chromatography; iii) satisfactory performance in terms of in-house validation results: linearity showed linear response between 2.5 and 50 mg/L; percentages of standard deviation related to intra-day and inter-day precision were lower than 15%; LOD and LOQ were lower than 1 and 1.5 mg/kg, respectively; in the case of the concentration of FAEEs closer to the legal limit, the recoveries of each analyte were higher than 94%; iv) no significant differences were evidenced for analytes of interest when the alternative procedure offline HPLC-GC-FID with PTV injector was applied to actual samples with low, medium and high content of FAEEs with respect to the official method.

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    Authors: Bonetta, Si; Pignata, C.; Porro, V.; Gilli, G.; +1 Authors
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    Authors: L. Pari; A. Scarfone; V. Alfano; S. Bergonzoli; +4 Authors

    In larga parte i canapicoltori italiani non riescono a sfruttare appieno le potenzialità della canapa per mancanza di tecnologie adatte alla raccolta e alla lavorazione dei vari prodotti. Sarà necessario quindi individuare tecnologie ad hoc per l’Italia al fine di incrementare la redditività della coltura.

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    Authors: Cornara D.; Marra M.; Tedone B.; Cavalieri V.; +3 Authors

    Le cicale sono insetti xilemomizi abbondanti negli oliveti in Puglia; determinare il loro ruolo nella trasmissione di Xylella fastidiosa è di cruciale importanza per la comprensione dell’epidemiologia del CoDiRO. I dati derivanti da tre anni di prove di infettività naturale e trasmissione suggeriscono che le cicale non hanno un ruolo nella trasmissione del batterio negli oliveti salentini.

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    Authors: Alessandro Sgobbo;

    Lo spostamento del core business della Risk Economy verso la prevenzione trova, nelle città mediterranee, un rilevante ostacolo nelle politiche di Austerity. Diventa pertanto essenziale che le limitate risorse disponibili siano utilizzate per l’implementazione di prodotti e best practices in grado di accompagnare gli effetti in resilienza ad altrettanto rilevanti risultati in termini di “inclusivitàˮ, servizi e qualità urbana. La sperimentazione condotta dall’autore nel progetto di Ricerca Metropolis dimostra che, per gli aspetti idraulici, l’approccio olistico alla complessità urbana connaturato nel “Water Sensitive Urban Planning” consente di coniugare più efficacemente tali concorrenti esigenze rispetto alle pratiche di “Water Sensitive Urban Design”. Occorre però che il pianificatore sia supportato da modelli previsionali in grado di valutare anche quantitativamente gli effetti resilienti delle soluzioni proposte. Parole chiave: resilienza, rigenerazione, pluvial flooding BDC. Bollettino Del Centro Calza Bini, V. 18 N. 1 (2018): Approaches and Tools for Implementing the Circular City Model

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    Authors: Palagano R.; Valli E.; Tura M.; Cevoli C.; +4 Authors

    INTRODUZIONE Come ben noto, il contenuto in etil esteri degli acidi grassi (EEAGs) è considerato un parametro diretto di qualità da applicarsi per definire l’appartenenza di oli vergini di oliva (VOOs) ad una specifica categoria (limite fissato a ≤35 mg/kg per la categoria extra vergine di oliva per la legislazione UE) ed è anche ritenuto un parametro indiretto utile per mettere in luce possibili miscele fraudolente tra un olio extra vergine di oliva ed un olio soft deodorato [1]. In questo lavoro è stato proposto un approccio analitico alternativo al metodo ufficiale con lo scopo di offrire una procedura non in linea HPLC-GC-FID migliora tiva in termini di tempo di analisi, volume di solventi e livello di complessità. Più nello specifico, è stato impiegato l’HPLC per collezionare i EEAGs dagli oli vergini di oliva (VOOs) al posto della tradizionale cromatografia liquida su colonna. Inoltre, è stato testato anche un iniettore di vaporizzazione a temperatura programmata (PTV) come possibile alternativa a quello on-column poichè il PTV è considerato più versatile consentendo il controllo programmato e l’ottimizzazione della modalità di iniezione (split/splitless). Le prestazioni del metodo alternativo sono state valutate controllando i risultati ottenuti per differenti parametri che caratterizzano il protocollo di validazione interna [2]. A tale scopo, sono state analizzate tipologie specifiche di campioni quali olio di oliva raffinato aggiunto di cinque concentrazioni diverse di standard puri degli analiti di interesse e campioni reali VOOs caratterizzati da concentrazioni diverse di EEAGs (basso, medio ed alto contenuto di EEAGs) ed applicando in parallelo i due metodi (alternativo vs ufficiale). INTRODUCTION As well known, the content in fatty acid ethyl esters (FAEEs) represents a direct quality parameter to define the quality grade of virgin olive oil (VOOs) (limit set at ≤35 mg/kg oil for extra virgin olive oil category for EU regulation), and it is also considered an indirect parameter to detect fraudulent mixtures of EVOO with lower quality oils such as soft deodorized olive oils [1]. In this work, the official method has been revised to propose an alternative off-line procedure based on HPLC-GC-FID, more suitable in terms of time of analysis, volume of solvents and level of complexity. Specifically, the analytical protocol proposed HPLC for collecting FAEEs from VOOs instead of the traditional liquid chromatography of the official method. Moreover, a programmed temperature vaporizer (PTV) injector was tested as an alternative to the on-column one because PTV is considered a more versatile option as the temperature control is time-programmed and the injection mode (split/splitless) can be optimized. The performance of the alternative method was evaluated, checking the results in terms of several parameters regarding in-house validation protocol [2]. For this control, specific kinds of samples as refined olive oil spiked with five concentrations of a pure standard of analytes of interest and real VOOs characterized by different contents of FAEEs (low, medium, high) were analyzed applying in parallel the two methods (alternative vs official) (see the Figure). RESULTS The main advantages of this revised protocol may be summarized as: i) significant reduction in time and solvents needed (reduction of more than 50% and 80% for time and solvents, respectively compared to the official procedure); ii) simplification of the separation step of FAEEs fraction avoiding the manual packing of the glass column for the liquid chromatography; iii) satisfactory performance in terms of in-house validation results: linearity showed linear response between 2.5 and 50 mg/L; percentages of standard deviation related to intra-day and inter-day precision were lower than 15%; LOD and LOQ were lower than 1 and 1.5 mg/kg, respectively; in the case of the concentration of FAEEs closer to the legal limit, the recoveries of each analyte were higher than 94%; iv) no significant differences were evidenced for analytes of interest when the alternative procedure offline HPLC-GC-FID with PTV injector was applied to actual samples with low, medium and high content of FAEEs with respect to the official method.

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    Authors: Bonetta, Si; Pignata, C.; Porro, V.; Gilli, G.; +1 Authors
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    Authors: L. Pari; A. Scarfone; V. Alfano; S. Bergonzoli; +4 Authors

    In larga parte i canapicoltori italiani non riescono a sfruttare appieno le potenzialità della canapa per mancanza di tecnologie adatte alla raccolta e alla lavorazione dei vari prodotti. Sarà necessario quindi individuare tecnologie ad hoc per l’Italia al fine di incrementare la redditività della coltura.

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    Authors: Cornara D.; Marra M.; Tedone B.; Cavalieri V.; +3 Authors

    Le cicale sono insetti xilemomizi abbondanti negli oliveti in Puglia; determinare il loro ruolo nella trasmissione di Xylella fastidiosa è di cruciale importanza per la comprensione dell’epidemiologia del CoDiRO. I dati derivanti da tre anni di prove di infettività naturale e trasmissione suggeriscono che le cicale non hanno un ruolo nella trasmissione del batterio negli oliveti salentini.

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    Authors: Alessandro Sgobbo;

    Lo spostamento del core business della Risk Economy verso la prevenzione trova, nelle città mediterranee, un rilevante ostacolo nelle politiche di Austerity. Diventa pertanto essenziale che le limitate risorse disponibili siano utilizzate per l’implementazione di prodotti e best practices in grado di accompagnare gli effetti in resilienza ad altrettanto rilevanti risultati in termini di “inclusivitàˮ, servizi e qualità urbana. La sperimentazione condotta dall’autore nel progetto di Ricerca Metropolis dimostra che, per gli aspetti idraulici, l’approccio olistico alla complessità urbana connaturato nel “Water Sensitive Urban Planning” consente di coniugare più efficacemente tali concorrenti esigenze rispetto alle pratiche di “Water Sensitive Urban Design”. Occorre però che il pianificatore sia supportato da modelli previsionali in grado di valutare anche quantitativamente gli effetti resilienti delle soluzioni proposte. Parole chiave: resilienza, rigenerazione, pluvial flooding BDC. Bollettino Del Centro Calza Bini, V. 18 N. 1 (2018): Approaches and Tools for Implementing the Circular City Model

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    https://doi.org/10.6092/2284-4...
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