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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: D'Attoma G.; Morelli M.; De La Fuente L.; Saponari M.; +2 Authors

    Xylella fastidiosa subsp. pauca strain ‘De Donno’ has been recently identified as the causal agent of a severe disease affecting olive trees in a wide area of the Apulia Region (Italy). While insights on the genetics and epidemiology of this virulent strain have been gained, the complex network of interactions with the main susceptible host remains to be explored. A fundamental tool for understanding such interactions is the development of bacterial mutants for functional analysis of genes involved in the host recognition, pathogenicity and insect transmission. Experimental studies have demonstrated natural competence of X. fastidiosa in the uptake of exogenous genetic material, a feature exploited for site-specific introduction or deletion of genes through homologous recombination. Nevertheless, numerous studies have shown that several factors may affect X. fastidiosa transformation efficiency, including growth rate, twitching motility, sequence similarity, and the presence of Restriction-Modification systems that cleave incoming DNA. On this basis, two different plasmids containing the chromosomal replication origin (oriC) of X. fastidiosa and E. coli were used to transform X. fastidiosa ‘De Donno’ in order to produce a GFP-expressing and a knockout strain for the rpfF gene, a crotonase producing a diffusible signal factor (DSF), involved in the quorum-sensing system. Repeated attempts to exploit natural competence, introducing the donor plasmids into X. fastidiosa ‘De Donno’ failed, highlighting the critical role of genetic diversity in recombination performances of this pathogen. Conversely, GFP and RpfF- mutants were successfully obtained by co-electroporation in the presence of an inhibitor of the Type I R-M system, that had been proved to impact the stable acquisition of foreign DNA by X. fastidiosa subsp. fastidiosa. Availability of mutants for one of the most virulent strains of X. fastidiosa opens for new explorations of host-microbe interactions, important to elucidate mechanisms underpinning the differential responses recorded upon infections of different olive cultivars and toward the implementation of strategies to mitigate the impact of the disease.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Other literature type . Article . Conference object . 2019
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Other literature type . Article . Conference object . 2019
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Fasma Diele; Carmela Marangi;

    We propose novel positive numerical integrators for approximating predator-prey models. The schemes are based on suitable symplectic procedures applied to the dynamical system written in terms of the log transformation of the original variables. Even if this approach is not new when dealing with Hamiltonian systems, it is of particular interest in population dynamics since the positivity of the approximation is ensured without any restriction on the temporal step size. When applied to separable M-systems, the resulting schemes are proved to be explicit, positive, Poisson maps. The approach is generalized to predator-prey dynamics which do not exhibit an M-system structure and successively to reaction-diffusion equations describing spatially extended dynamics. A classical polynomial Krylov approximation for the diffusive term joint with the proposed schemes for the reaction, allows us to propose numerical schemes which are explicit when applied to well established ecological models for predator-prey dynamics. Numerical simulations show that the considered approach provides results which outperform the numerical approximations found in recent literature.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Discrete & Continuou...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Discrete & Continuou...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Marco Morabito; Alessandro Messeri; Pascal Noti; Ana Casanueva; +8 Authors

    platform (https://heatshield.zonalab.it/) operating for Europe and developed within the framework of the HEAT-SHIELD project. This system is based on probabilistic medium-range forecasts calibrated on approximately 1800 meteorological stations in Europe and provides the ensemble forecast of the daily maximum heat stress. The platform provides a non-customized output represented by a map showing the weekly maximum probability of exceeding a specific heat stress condition, for each of the four upcoming weeks. Customized output allows the forecast of the personalized local heat-stress-risk based on workers&rsquo physical, clothing and behavioral characteristics and the work environment (outdoors in the sun or shade), also taking into account heat acclimatization. Personal daily heat stress risk levels and behavioral suggestions (hydration and work breaks recommended) to be taken into consideration in the short term (5 days) are provided together with long-term heat risk forecasts (up to 46 days), all which are useful for planning work activities. The HEAT-SHIELD platform provides adaptation strategies for &ldquo health warning systems focus on warning vulnerable groups in order to reduce mortality. However, human health and performance are affected at much lower environmental heat strain levels than those directly associated with higher mortality. Moreover, workers are at elevated health risks when exposed to prolonged heat. This study describes the multilingual &ldquo HEAT-SHIELD occupational warning system&rdquo the impact of global warming. Existing heat&ndash managing&rdquo

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ International Journa...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2019
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    UCrea
    Other literature type . 2019
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ International Journa...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      UCrea
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Teresa Amaro; Gian Marco Luna; Roberto Danovaro; David S.M. Billett; +1 Authors

    Sediments in the Nazare Canyon (NE Atlantic) are inhabited by unexpectedly high abundances of the deposit-feeding holothurian Molpadia musculus. The energetic demand of such a large megafaunal biomass is presumably high and requires the efficient exploitation of the food inputs coming from the photic zone. We hypothesise the existence of cooperative interactions between these deep-sea holothurians and prokaryotes in their guts. To investigate these interactions, sediment samples and holothurians were collected at ca. 3500 m depth using a Remote Operated Vehicle (ROV) and an incubation chamber used to "harvest" faeces from the holothurian. In all of these samples (ingested sediment from different sectors of the holothurian gut content, faeces and sediments) we determined total prokaryotic abundance, the relative abundance of Bacteria and Archaea (by means of Catalysed Reporter Deposition-Fluorescence in situ Hybridisation) and bacterial diversity (by means of fingerprinting techniques: ARISA and T-RFLP). Prokaryotic abundances and bacterial diversity in the holothurian gut were very high (up to 10(5) bacterial Operational Taxonomic Units) and significantly greater than in surrounding bottom sediments. Archaea represented a key component within the gut of the holothurians and in certain tracts dominated the prokaryotic assemblage. We also found that ca. 40% of bacterial OTUs were associated uniquely with the gut contents (i.e., absent in surrounding sediments). These findings suggest the occurrence of wide and highly diversified interactions between prokaryotes and deep-sea holothurians. Results presented here provide new insights into the potential relationships between deep-sea holothurians and specific associations of Archaea and Bacteria within their guts. The work opens new perspectives for investigating the diversity of prokaryotes associated to deep-sea megafauna. (C) 2012 Elsevier Ltd. All rights reserved.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Max Craglia; Jiri Hradec; Stefano Nativi; Mattia Santoro;

    Big Earth Data-Cube infrastructures are becoming more and more popular to provide Analysis Ready Data, especially for managing satellite time series. These infrastructures build on the concept of multidimensional data model (data hypercube) and are complex systems engaging different disciplines and expertise. For this reason, their interoperability capacity has become a challenge in the Global Change and Earth System science domains. To address this challenge, there is a pressing need in the community to reach a widely agreed definition of Data-Cube infrastructures and their key features. In this respect, a discussion has started recently about the definition of the possible facets characterizing a Data-Cube in the Earth Observation domain. This manuscript contributes to such debate by introducing a view-based model of Earth Data-Cube systems to design its infrastructural architecture and content schemas, with the final goal of enabling and facilitating interoperability. It introduces six modeling views, each of them is described according to: its main concerns, principal stakeholders, and possible patterns to be used. The manuscript considers the Business Intelligence experience with Data Warehouse and multidimensional "cubes" along with the more recent and analogous development in the Earth Observation domain, and puts forward a set of interoperability recommendations based on the modeling views.

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    Big Earth Data
    Article . 2017 . Peer-reviewed
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    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    CNR ExploRA
    Article . 2017
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Big Earth Dataarrow_drop_down
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      Big Earth Data
      Article . 2017 . Peer-reviewed
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    Authors: Ana, Popovic; Tran, Hai; Anatoly, Tchigvintsev; Mahbod, Hajighasemi; +17 Authors

    AbstractMetagenomics has made accessible an enormous reserve of global biochemical diversity. To tap into this vast resource of novel enzymes, we have screened over one million clones from metagenome DNA libraries derived from sixteen different environments for carboxylesterase activity and identified 714 positive hits. We have validated the esterase activity of 80 selected genes, which belong to 17 different protein families including unknown and cyclase-like proteins. Three metagenomic enzymes exhibited lipase activity, and seven proteins showed polyester depolymerization activity against polylactic acid and polycaprolactone. Detailed biochemical characterization of four new enzymes revealed their substrate preference, whereas their catalytic residues were identified using site-directed mutagenesis. The crystal structure of the metal-ion dependent esterase MGS0169 from the amidohydrolase superfamily revealed a novel active site with a bound unknown ligand. Thus, activity-centered metagenomics has revealed diverse enzymes and novel families of microbial carboxylesterases, whose activity could not have been predicted using bioinformatics tools.

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    Europe PubMed Central
    Article . 2017
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    Authors: Incarbona A; Sprovieri M; Di Stefano A; Di Stefano E; +5 Authors

    The study of planktonic organisms during abrupt climatic variations of the last glacial period (Dansgaard-Oeschger oscillations, D-O) may reveal important insights on climatic, oceanographic and biological interactions. Here we present planktic foraminifera and coccolithophore data collected at the Ocean Drilling Program Site 963 (Sicily Channel), with a mean sampling resolution of respectively 43.5 and 98.9. yr, over the interval between 70,000 and 20,000. yr ago. The paleoenvironmental reconstruction suggests that three different scenarios can be seen across each D-O cycle: 1. oligotrophic surface water and a deep thermocline for the early Interstadials; 2. a Deep Chlorophyll Maximum and coccolithophore winter/spring blooming in the late Interstadials; 3. reduced productivity together with the shallowing of the nutricline depth during Stadials and Heinrich events. The unique mode of productivity dynamics is corroborated by comparing our paleoecological results with those published from high-resolution cores in the Alboran Sea clearly indicating reduced trophic levels during Stadials and Heinrich events. Finally, we argue that the density contrast between the Atlantic water inflow and subsurface water may have affected productivity dynamics in such a large area. The strong vertical density gradient may have hampered the vertical convection of the water column, producing a negative effect on biological productivity, especially during Stadial phases. © 2013 Elsevier B.V.

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    Authors: Laura Carnieletto; Borja Badenes; Marco Belliardi; Adriana Bernardi; +9 Authors

    [EN] The design of ground source heat pumps is a fundamental step to ensure the high energy efficiency of heat pump systems throughout their operating years. To enhance the diffusion of ground source heat pump systems, two different tools are developed in the H2020 research project named, Cheap GSHPs: A design tool and a decision support system. In both cases, the energy demand of the buildings may not be calculated by the user. The main input data, to evaluate the size of the borehole heat exchangers, is the building energy demand. This paper presents a methodology to correlate energy demand, building typologies, and climatic conditions for different types of residential buildings. Rather than envelope properties, three insulation levels have been considered in different climatic conditions to set up a database of energy profiles. Analyzing European climatic test reference years, 23 locations have been considered. For each location, the overall energy and the mean hourly monthly energy profiles for heating and cooling have been calculated. Pre-calculated profiles are needed to size generation systems and, in particular, ground source heat pumps. For this reason, correlations based on the degree days for heating and cooling demand have been found in order to generalize the results for different buildings. These correlations depend on the Koppen-Geiger climate scale. This work received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 657982.

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    Authors: Michele De Carli; Adriana Bernardi; Matteo Cultrera; Giorgia Dalla Santa; +10 Authors

    Weather plays an important role for energy uses in buildings. For this reason, it is required to define the proper boundary conditions in terms of the different parameters affecting energy and comfort in buildings. They are also the basis for determining the ground temperature in different locations, as well as for determining the potential for using geothermal energy. This paper presents a database for climates in Europe that has been used in a freeware tool developed as part of the H2020 research project named “Cheap-GSHPs”. The standard Köppen-Geiger climate classification has been matched with the weather data provided by the ENERGYPLUS and METEONORM software database. The Test Reference Years of more than 300 locations have been considered. These locations have been labelled according to the degree-days for heating and cooling, as well as by the Köppen-Geiger scale. A comprehensive data set of weather conditions in Europe has been created and used as input for a GSHP sizing software, helping the user in selecting the weather conditions closest to the location of interest. The proposed method is based on lapse rates and has been tested at two locations in Switzerland and Ireland. It has been demonstrated as quite valid for the project purposes, considering the spatial distribution and density of available data and the lower computing load, in particular for locations where altitude is the main factor controlling on the temperature variations.

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    Authors: Kutiel H.; M. Brunetti;

    Abstract. The impact of the upper level (500 hPa) teleconnection between the North-Sea and the Caspian (NCP) on the temperature and precipitation regimes in the Eastern Mediterranean (EM) have been studied and reported and an index (NCPI) that measures the normalized geopotential heights' differences between the two poles of this teleconnection has been defined. In the present study, the impact of the NCP on the temperature regime over the entire European continent is presented. In particular, the correlation between temperature and the NCPI has been evaluated, on a monthly basis, over the entire Euro-Mediterranean domain for the 1948–2007 period. The results highlight a significant positive correlation in the north-western area of the domain and a significant negative correlation in the south-eastern one. These two poles were also highlighted by comparing the temperature anomalies associated with both phases of NCP. The importance of this sort of NCP-induced temperature bi-pole in the context of temperature variability over Europe and the Mediterranean has been evaluated by applying a Principal Component Analysis to the temperature dataset. The results showed that the temperature bi-pole is associated with the second most important mode of temperature variability over the domain, but if the analysis is restricted to the months associated to NCP (+) and NCP (−), it becomes the first mode with 29.2 % of associated variance.

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    Natural Hazards and Earth System Sciences
    Article . 2011 . Peer-reviewed
    License: CC BY
    Data sources: Crossref
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    Article . 2011
    Data sources: CNR ExploRA
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    Conference object . 2012
    Data sources: CNR ExploRA
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Natural Hazards and ...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Natural Hazards and Earth System Sciences
      Article . 2011 . Peer-reviewed
      License: CC BY
      Data sources: Crossref
      CNR ExploRA
      Article . 2011
      Data sources: CNR ExploRA
      CNR ExploRA
      Conference object . 2012
      Data sources: CNR ExploRA
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    Authors: D'Attoma G.; Morelli M.; De La Fuente L.; Saponari M.; +2 Authors

    Xylella fastidiosa subsp. pauca strain ‘De Donno’ has been recently identified as the causal agent of a severe disease affecting olive trees in a wide area of the Apulia Region (Italy). While insights on the genetics and epidemiology of this virulent strain have been gained, the complex network of interactions with the main susceptible host remains to be explored. A fundamental tool for understanding such interactions is the development of bacterial mutants for functional analysis of genes involved in the host recognition, pathogenicity and insect transmission. Experimental studies have demonstrated natural competence of X. fastidiosa in the uptake of exogenous genetic material, a feature exploited for site-specific introduction or deletion of genes through homologous recombination. Nevertheless, numerous studies have shown that several factors may affect X. fastidiosa transformation efficiency, including growth rate, twitching motility, sequence similarity, and the presence of Restriction-Modification systems that cleave incoming DNA. On this basis, two different plasmids containing the chromosomal replication origin (oriC) of X. fastidiosa and E. coli were used to transform X. fastidiosa ‘De Donno’ in order to produce a GFP-expressing and a knockout strain for the rpfF gene, a crotonase producing a diffusible signal factor (DSF), involved in the quorum-sensing system. Repeated attempts to exploit natural competence, introducing the donor plasmids into X. fastidiosa ‘De Donno’ failed, highlighting the critical role of genetic diversity in recombination performances of this pathogen. Conversely, GFP and RpfF- mutants were successfully obtained by co-electroporation in the presence of an inhibitor of the Type I R-M system, that had been proved to impact the stable acquisition of foreign DNA by X. fastidiosa subsp. fastidiosa. Availability of mutants for one of the most virulent strains of X. fastidiosa opens for new explorations of host-microbe interactions, important to elucidate mechanisms underpinning the differential responses recorded upon infections of different olive cultivars and toward the implementation of strategies to mitigate the impact of the disease.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
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    Conference object . 2019
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    Other literature type . Article . Conference object . 2019
    License: CC BY
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    Conference object . 2019
    Data sources: CNR ExploRA
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      ZENODO
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      Data sources: Datacite
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      ZENODO
      Other literature type . Article . Conference object . 2019
      License: CC BY
      CNR ExploRA
      Conference object . 2019
      Data sources: CNR ExploRA
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      This Research product is the result of merged Research products in OpenAIRE.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/