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  • SDSN - Greece
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Yahia M. N. D.; Yahia O. K. -D.; Gueroun S. K. M.; Aissi M.; +3 Authors

    The alien Erythraean jellyfish Rhopilema nomadica was first recorded in Tunisia waters (Gulf of Gabes) in 2008. Subsequently it was sighted in the Bizerte Channel and Gulf of Tunis where it has been regularly observed since 2010 during summer and autumn months. peer-reviewed

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    Article . 2013 . Peer-reviewed
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    BioInvasions Records
    Other literature type . Article . 2013 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ OAR@UMarrow_drop_down
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      OAR@UM
      Article . 2013 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      BioInvasions Records
      Other literature type . Article . 2013 . Peer-reviewed
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    Authors: Di Camillo, Cristina G.; Ponti, Massimo; Bavestrello, Giorgio; Krzelj, Maja; +1 Authors

    In the Mediterranean, habitat-forming corals often characterize essential fish habitats. While their distribution is sufficiently known for the western basin, few data are available from the Central-Eastern Mediterranean Sea (CEM). This study fills this gap supplying the largest dataset ever built on the geographical and bathymetric distribution of the most relevant habitat-forming corals (Eunicella cavolini, Eunicella verrucosa, Eunicella singularis, Leptogorgia sarmentosa, Paramuricea clavata, Corallium rubrum and Savalia savaglia) of the CEM. Information collected from different sources such as literature, citizen science, and from the World Wide Web (WWW) was combined. Videos published on the WWW provided additional information on the presence of fishing lines and signs of damage, as well as on the distribution of purple and yellow-purple colonies of Paramuricea clavata. The study highlighted the impressive amount of information that the WWW can offer to scientists, termed here as Web Ecological Knowledge (WEK). The WEK is constantly fuelled by internauts, representing a free, refreshable, long-term exploitable reservoir of information. A quick and easy method to retrieve data from the WWW was illustrated. In addition, the distribution of corals was overlapped to marine protected areas and to the distribution of environmental conditions suitable for coralligenous habitats, fragile biogenic Mediterranean structures hosting complex assemblages in need of strict protection. The collected data allowed identifying priority areas with high species diversity and sites that are impacted by fishing activities. Supplied data can correctly address conservation and restoration policies in the CEM, adding an important contribution to ecosystem-based marine spatial planning.

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    Article . 2017
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    Biodiversity and Conservation
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    Biodiversity and Conservation
    Other literature type . Article . 2017 . 2018 . Peer-reviewed
    License: Springer TDM
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      Biodiversity and Conservation
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      Biodiversity and Conservation
      Other literature type . Article . 2017 . 2018 . Peer-reviewed
      License: Springer TDM
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Severin D. H. Irl; Andreas H. Schweiger; Félix M. Medina; José María Fernández-Palacios; +5 Authors

    [Main conclusion] Both AE and SIE rarity on La Palma show a clear spatial pattern, with hotspots of endemic rarity found at high elevations and in rare climates, presumably owing to geographical and climatic constraints and possibly anthropogenic pressure (e.g., land use, introduced herbivores, fire). Areas of high rarity estimates coincide with the distribution and extent of PAs on La Palma, especially since the recent addition of the Natura 2000 sites. The hypervolume approach is a promising tool to estimate species range sizes, and can be applied on all scales where point/plot data are available. [Methods] We recorded all present endemic vascular plant species in 1,212 plots covering the entire island. We calculated endemic rarity (corrected range-rarity richness for endemics) using a rarity estimation approach based on kernel density estimations (hypervolume approach). We performed a sensitivity analysis based on multiple linear regressions and relative importance estimations of environmental drivers to estimate the performance of the hypervolume-based rarity estimation compared to standard methods (occurrence frequency, convex hulls, alpha hulls). [Aim] Rarity—an important measure for conservation biogeography—can vary over many orders of magnitude. However, it is unclear which regional-scale abiotic conditions drive processes affecting rarity of endemic species on islands. To support conservation efforts, we (1) assess the main abiotic drivers of endemic rarity, (2) determine how well existing protected areas (PAs) coincide with hotspots of endemic rarity and (3) introduce and evaluate a new hypervolume-based rarity estimator. [Results] Climate variables (mean annual temperature, climatic rarity, precipitation variability) best explained archipelago endemic (AE) and single-island endemic (SIE) rarity. Existing PAs covered the majority of AE and SIE rarity, especially national and natural parks as well as the Natura 2000 sites. In our study system, hypervolumes performed better than standard measures of range size. This work contributed to and was partially supported by the European H2020 Project 641762 ECOPOTENTIAL: Improving future ecosystem benefits through Earth Observations. [Location] La Palma (Canary Islands). © 2017 The Authors. Peer Reviewed

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    Diversity and Distributions
    Other literature type . Article . 2017 . Peer-reviewed
    License: CC BY
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      Other literature type . Article . 2017 . Peer-reviewed
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    Authors: Menconi V.; Gustinelli A.; Caffara M.; Francalacci C.; +1 Authors

    L’anisakiasi è una zoonosi parassitaria di origine alimentare sostenuta da larve al terzo stadio (L3) di nematodi appartenenti alla famiglia Anisakidae ed in particolare al genere Anisakis. Soprattutto in seguito alla recente emergenza di sindromi allergiche da Anisakis, l’EFSA nel 2010 ha pubblicato un parere scientifico sui rischi correlati alla presenza di parassiti zoonotici in prodotti ittici, considerando sicura la pratica d’allevamento del salmone atlantico (Salmo salar) in quanto, se allevati partendo da avannotti prodotti in sistemi chiusi e alimentati sempre con mangime inerte, questi animali presenterebbero un rischio di infestazione da larve di Anisakis trascurabile. Su questa base, il Reg. UE n. 1276/2011, che modifica l’allegato III del Reg. CE n. 853\2004, puntualizza la possibile deroga al trattamento di congelamento, obbligatorio per i prodotti della pesca destinati ad essere consumati sotto forma di preparazioni a rischio (crudi, marinati, affumicati a freddo, ecc.), per i prodotti ittici derivanti da allevamento quando vengono rispettati i criteri definiti dall’EFSA analogamente al salmone atlantico. Appare quindi d’importanza strategica fornire dati scientifici estesi ed attendibili sull’assenza di parassiti zoonotici nei prodotti della maricoltura mediterranea non solo ai fini di una loro migliore qualificazione commerciale ma anche per una più facile applicabilità di tale deroga da parte delle autorità competenti. Nell’ambito delle attività di ricerca previste dal progetto europeo H2020 ParaFishControl “Advanced Tools and Research Strategies for Parasite Control in European farmed fish”, da marzo 2016 a giugno 2017 è stato condotto un esteso monitoraggio parassitologico in spigole (Dicentrarchus labrax) ed orate (Sparus aurata) allevate in Italia. Le attività di campionamento hanno coinvolto 4 allevamenti nazionali, di cui 3 in gabbie galleggianti ed uno in vasche a terra, prelevando almeno 258 esemplari per specie/allevamento suddivisi in 4 campionamenti a cadenza stagionale per un totale di 1040 spigole e 1090 orate. La numerosità campionaria è stata calcolata in modo da ottenere almeno il 95% di probabilità di evidenziare un esemplare positivo con prevalenza >2%. Per la ricerca di larve di nematodi Anisakidae sono stati associati: esame visivo diretto e mediante transilluminatore di visceri e porzioni muscolari, metodo della UV-press dei filetti e digestione con soluzione cloro- peptica del pacchetto viscerale. Tutti gli esemplari di spigola e orata esaminati sono risultati negativi per la presenza di stadi larvali di Anisakis spp. In un unico esemplare di spigola proveniente da un allevamento in gabbia è stata evidenziata la presenza di una larva di Hysterothylacium fabri (nematode non zoonotico) incistata sulla superficie del fegato ed identificata a livello di specie mediante PCR-RFLP. Alla luce della trasmissione di questo parassita a pesci predatori per via trofica, similmente ai nematodi Anisakidae zoonotici, nel prossimo futuro verranno condotte indagini mirate ad identificare i fattori di rischio che hanno condotto all’infestazione da H. fabri in allevamento, seppur in un unico esemplare, ed individuare gli eventuali punti critici da monitorare. I risultati ottenuti da questa indagine indicano comunque come nelle spigole e nelle orate allevate in Italia il rischio d’infestazione da Anisakis sia da ritenersi trascurabile, aprendo la via ad azioni di qualificazione dei prodotti della maricoltura mediterranea.

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    Authors: Teresa Amaro; Gian Marco Luna; Roberto Danovaro; David S.M. Billett; +1 Authors

    Sediments in the Nazare Canyon (NE Atlantic) are inhabited by unexpectedly high abundances of the deposit-feeding holothurian Molpadia musculus. The energetic demand of such a large megafaunal biomass is presumably high and requires the efficient exploitation of the food inputs coming from the photic zone. We hypothesise the existence of cooperative interactions between these deep-sea holothurians and prokaryotes in their guts. To investigate these interactions, sediment samples and holothurians were collected at ca. 3500 m depth using a Remote Operated Vehicle (ROV) and an incubation chamber used to "harvest" faeces from the holothurian. In all of these samples (ingested sediment from different sectors of the holothurian gut content, faeces and sediments) we determined total prokaryotic abundance, the relative abundance of Bacteria and Archaea (by means of Catalysed Reporter Deposition-Fluorescence in situ Hybridisation) and bacterial diversity (by means of fingerprinting techniques: ARISA and T-RFLP). Prokaryotic abundances and bacterial diversity in the holothurian gut were very high (up to 10(5) bacterial Operational Taxonomic Units) and significantly greater than in surrounding bottom sediments. Archaea represented a key component within the gut of the holothurians and in certain tracts dominated the prokaryotic assemblage. We also found that ca. 40% of bacterial OTUs were associated uniquely with the gut contents (i.e., absent in surrounding sediments). These findings suggest the occurrence of wide and highly diversified interactions between prokaryotes and deep-sea holothurians. Results presented here provide new insights into the potential relationships between deep-sea holothurians and specific associations of Archaea and Bacteria within their guts. The work opens new perspectives for investigating the diversity of prokaryotes associated to deep-sea megafauna. (C) 2012 Elsevier Ltd. All rights reserved.

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    Authors: Eniko Kadar; Andrew Fisher; Björn Stolpe; Roy M. Harrison; +2 Authors

    We report on metal enrichment along a natural pH gradient owing to increased CO2 degassing at cold, shallow seeps of Vulcano Island in the Mediterranean Sea, off Sicily. We assessed composition of unfiltered and filtered seawater (<100 nm) along acidic zones ranging between ambient and pH 5, and showed that most seep derived elements are present as nanoclusters which then aggregate into larger colloids while mixing with ambient seawater along a pH gradient. Size and elemental composition of such naturally occurring nanoparticles assessed by modern characterisation methods were in good agreement with the results from conventional analytical methods. We provide analytical evidence for the presence in the water column of a large fraction of seep derived elements (e.g. approximately 50% of iron, over 80% of Mn, 100% of Cr, S and Zn) in the form of nano sized particles (e.g. <100 nm) even at typical open ocean pHs. We launch in situ sampling protocols and sample preparation procedures for multi-method suitable to obtain accurate measurements on nanoparticles from environmental samples. Based on our results a first insight to the formation of natural nanoparticles at cold CO2 seeps is presented and the persistence of such nano-clusters in the surrounding seawater is stipulated.

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    Marine Chemistry
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    Other literature type . Article . 2012 . Peer-reviewed
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      Marine Chemistry
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      Marine Chemistry
      Other literature type . Article . 2012 . Peer-reviewed
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    Authors: Jaume, Pérez-Sánchez; Genciana, Terova; Paula, Simó-Mirabet; Simona, Rimoldi; +4 Authors

    The skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. More than 2,000 proteins were identified with a protein score filter of 30. The identified proteins were represented in 418 canonical pathways of the Ingenuity Pathway software. After filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. The mitochondrial cluster contained 59 proteins related to oxidative phosphorylation and mitochondrial dysfunction. The second cluster contained 79 proteins related to antigen presentation and protein ubiquitination pathways. The third cluster contained 257 proteins where proteins related to protein synthesis, cellular assembly, and epithelial integrity were over-represented. The latter group also included acute phase response signaling. In parallel, two-dimensional gel electrophoresis methodology identified six proteins spots of different protein abundance when comparing unstressed fish with chronically stressed fish in an experimental model that mimicked daily farming activities. The major changes were associated with a higher abundance of cytokeratin 8 in the skin mucus proteome of stressed fish, which was confirmed by immunoblotting. Thus, the increased abundance of markers of skin epithelial turnover results in a promising indicator of chronic stress in fish.

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    Europe PubMed Central
    Article . 2017
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    Authors: Max Craglia; Jiri Hradec; Stefano Nativi; Mattia Santoro;

    Big Earth Data-Cube infrastructures are becoming more and more popular to provide Analysis Ready Data, especially for managing satellite time series. These infrastructures build on the concept of multidimensional data model (data hypercube) and are complex systems engaging different disciplines and expertise. For this reason, their interoperability capacity has become a challenge in the Global Change and Earth System science domains. To address this challenge, there is a pressing need in the community to reach a widely agreed definition of Data-Cube infrastructures and their key features. In this respect, a discussion has started recently about the definition of the possible facets characterizing a Data-Cube in the Earth Observation domain. This manuscript contributes to such debate by introducing a view-based model of Earth Data-Cube systems to design its infrastructural architecture and content schemas, with the final goal of enabling and facilitating interoperability. It introduces six modeling views, each of them is described according to: its main concerns, principal stakeholders, and possible patterns to be used. The manuscript considers the Business Intelligence experience with Data Warehouse and multidimensional "cubes" along with the more recent and analogous development in the Earth Observation domain, and puts forward a set of interoperability recommendations based on the modeling views.

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    Big Earth Data
    Article . 2017 . Peer-reviewed
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    Big Earth Data
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    CNR ExploRA
    Article . 2017
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    Authors: Ana, Popovic; Tran, Hai; Anatoly, Tchigvintsev; Mahbod, Hajighasemi; +17 Authors

    AbstractMetagenomics has made accessible an enormous reserve of global biochemical diversity. To tap into this vast resource of novel enzymes, we have screened over one million clones from metagenome DNA libraries derived from sixteen different environments for carboxylesterase activity and identified 714 positive hits. We have validated the esterase activity of 80 selected genes, which belong to 17 different protein families including unknown and cyclase-like proteins. Three metagenomic enzymes exhibited lipase activity, and seven proteins showed polyester depolymerization activity against polylactic acid and polycaprolactone. Detailed biochemical characterization of four new enzymes revealed their substrate preference, whereas their catalytic residues were identified using site-directed mutagenesis. The crystal structure of the metal-ion dependent esterase MGS0169 from the amidohydrolase superfamily revealed a novel active site with a bound unknown ligand. Thus, activity-centered metagenomics has revealed diverse enzymes and novel families of microbial carboxylesterases, whose activity could not have been predicted using bioinformatics tools.

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    Europe PubMed Central
    Article . 2017
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    Authors: Incarbona A; Sprovieri M; Di Stefano A; Di Stefano E; +5 Authors

    The study of planktonic organisms during abrupt climatic variations of the last glacial period (Dansgaard-Oeschger oscillations, D-O) may reveal important insights on climatic, oceanographic and biological interactions. Here we present planktic foraminifera and coccolithophore data collected at the Ocean Drilling Program Site 963 (Sicily Channel), with a mean sampling resolution of respectively 43.5 and 98.9. yr, over the interval between 70,000 and 20,000. yr ago. The paleoenvironmental reconstruction suggests that three different scenarios can be seen across each D-O cycle: 1. oligotrophic surface water and a deep thermocline for the early Interstadials; 2. a Deep Chlorophyll Maximum and coccolithophore winter/spring blooming in the late Interstadials; 3. reduced productivity together with the shallowing of the nutricline depth during Stadials and Heinrich events. The unique mode of productivity dynamics is corroborated by comparing our paleoecological results with those published from high-resolution cores in the Alboran Sea clearly indicating reduced trophic levels during Stadials and Heinrich events. Finally, we argue that the density contrast between the Atlantic water inflow and subsurface water may have affected productivity dynamics in such a large area. The strong vertical density gradient may have hampered the vertical convection of the water column, producing a negative effect on biological productivity, especially during Stadial phases. © 2013 Elsevier B.V.

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    Authors: Yahia M. N. D.; Yahia O. K. -D.; Gueroun S. K. M.; Aissi M.; +3 Authors

    The alien Erythraean jellyfish Rhopilema nomadica was first recorded in Tunisia waters (Gulf of Gabes) in 2008. Subsequently it was sighted in the Bizerte Channel and Gulf of Tunis where it has been regularly observed since 2010 during summer and autumn months. peer-reviewed

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    OAR@UM
    Article . 2013 . Peer-reviewed
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    BioInvasions Records
    Other literature type . Article . 2013 . Peer-reviewed
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      BioInvasions Records
      Other literature type . Article . 2013 . Peer-reviewed
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    Authors: Di Camillo, Cristina G.; Ponti, Massimo; Bavestrello, Giorgio; Krzelj, Maja; +1 Authors

    In the Mediterranean, habitat-forming corals often characterize essential fish habitats. While their distribution is sufficiently known for the western basin, few data are available from the Central-Eastern Mediterranean Sea (CEM). This study fills this gap supplying the largest dataset ever built on the geographical and bathymetric distribution of the most relevant habitat-forming corals (Eunicella cavolini, Eunicella verrucosa, Eunicella singularis, Leptogorgia sarmentosa, Paramuricea clavata, Corallium rubrum and Savalia savaglia) of the CEM. Information collected from different sources such as literature, citizen science, and from the World Wide Web (WWW) was combined. Videos published on the WWW provided additional information on the presence of fishing lines and signs of damage, as well as on the distribution of purple and yellow-purple colonies of Paramuricea clavata. The study highlighted the impressive amount of information that the WWW can offer to scientists, termed here as Web Ecological Knowledge (WEK). The WEK is constantly fuelled by internauts, representing a free, refreshable, long-term exploitable reservoir of information. A quick and easy method to retrieve data from the WWW was illustrated. In addition, the distribution of corals was overlapped to marine protected areas and to the distribution of environmental conditions suitable for coralligenous habitats, fragile biogenic Mediterranean structures hosting complex assemblages in need of strict protection. The collected data allowed identifying priority areas with high species diversity and sites that are impacted by fishing activities. Supplied data can correctly address conservation and restoration policies in the CEM, adding an important contribution to ecosystem-based marine spatial planning.

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    Biodiversity and Conservation
    Other literature type . Article . 2017 . 2018 . Peer-reviewed
    License: Springer TDM
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      Biodiversity and Conservation
      Other literature type . Article . 2017 . 2018 . Peer-reviewed
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