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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Tatjana Bakran-Petricioli; Dajana Kujundžić; Martina Naranđa; Donat Petricioli; +2 Authors

    In the last few years, the endemic Mediterranean bivalve Pinna nobilis has been exposed to dramatic mortality in its entire area, which could lead to the extinction of the species. Throughout the Mediterranean, a lot of effort is being put into finding ways of preserving it. One of the methods used to monitor recruitment and juveniles’ survival is the installation of collectors for bivalve larvae. We installed collectors at two locations: in Brijuni National Park (North Adriatic) and Luka Cove (central Adriatic). Our aim was to compare the fouling community on the collectors in two consecutive years (2019 and 2020), especially because the installation of collectors in 2020 coincided with mass mortality events of P. nobilis in the area. The number and size of juvenile P. nobilis and the qualitative and quantitative composition of the fouling communities were determined. The results show a reduction in the number and size of juvenile P. nobilis and an explosion of the invasive bivalve Anadara transversa population on collectors in the second year. In Luka Cove, another invasive species—the ascidian Styela plicata—also seriously affected other organisms on the collectors to the point of preventing analysis of the fouling community.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Marine Sc...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Marine Sc...arrow_drop_down
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    Authors: Márcio A. G. Coelho; Jean-Baptiste Ledoux; Joana Boavida; Diogo Paulo; +9 Authors

    The Gray's sea fan, Paramuricea grayi (Johnson, 1861), typically inhabits deep littoral and circalittoral habitats of the eastern temperate and tropical Atlantic Ocean. Along the Iberian Peninsula, where P. grayi is a dominant constituent of circalittoral coral gardens, two segregating lineages (yellow and purple morphotypes) were recently identified using single-copy nuclear orthologues. The mitochondrial genomes of 9 P. grayi individuals covering both color morphotypes were assembled from RNA-seq data, using samples collected at three sites in southern (Sagres and Tavira) and western (Cape Espichel) Portugal. The complete circular mitogenome is 18,668 bp in length, has an A + T-rich base composition (62.5%) and contains the 17 genes typically found in Octocorallia: 14 protein-coding genes (atp6, atp8, cob, cox1-3, mt-mutS, nad1-6, and nad4L), the small and large subunit rRNAs (rns and rnl), and one transfer RNA (trnM). The mitogenomes were nearly identical for all specimens, though we identified a noteworthy polymorphism (two SNPs 9 bp apart) in the mt-mutS of one purple individual that is shared with the sister species P. clavata. The mitogenomes of the two species have a pairwise sequence identity of 99.0%, with nad6 and mt-mutS having the highest rates of non-synonymous substitutions. info:eu-repo/semantics/publishedVersion MAR-01.04.02-FEAMP-0018 DivRestore/0013/2020 LA/P/0101/2020 FA_06_2017_045

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Mitochondrial DNA Pa...arrow_drop_down
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    Mitochondrial DNA Part B
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Mitochondrial DNA Pa...arrow_drop_down
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      Mitochondrial DNA Part B
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Authors: Ana Ilić; Ivana Rumbak; Ružica Brečić; Irena Colić Barić; +1 Authors

    Insufficient consumption of fruit and vegetables was found in primary school children. To address this problem, a three-year school-based multicomponent intervention was conducted in 14 primary schools in the City of Zagreb. The aim of the study was therefore to evaluate one of the primary goals of the intervention—the increase in fruit and vegetable intake among primary school children. A total of 681 children were allocated to the intervention (n = 300 in the control group and n = 381 in the intervention group). The intervention included 23 interactive classroom workshops, 10 cross-curricular activities, 13 homework challenges, visual exposure with educational posters in classrooms, parent education via the website, and the implementation of new dishes into the school food system. Fruit and vegetable intake was assessed using a semi-quantitative food frequency questionnaire at baseline and after the intervention. Data were analyzed by per-protocol analysis. The study involved 259 children (50.2% girls; age 7.7 ± 0.4 years; n = 116 in the control group and n = 143 in the intervention group) who completed a food intake frequency questionnaire at both time points. Children in the intervention group showed a significant increase (p < 0.001) in total daily fruit and vegetable intake (before: 332.1 ± 164.9 g; after: 430.1 ± 186.7 g) compared to the control group (before: 350.2 ± 187.5; after: 382.6 ± 196.8) after the intervention. The increase in fruit and vegetable intake was achieved in 89% of children, while 25% more children reached the daily recommendation of 400 g. The use of the multicomponent intervention showed potential to increase fruit and vegetable intake in primary school children.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Nutrients; Croatian ...arrow_drop_down
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    Nutrients
    Article . 2022
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    Nutrients
    Article . 2022
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      Nutrients
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      Nutrients
      Article . 2022
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Ana Ilić; Martina Bituh; Ružica Brečić; Irena Colić Barić;

    Objective: To assess differences in the amount of plate waste (PW) and food preferences among food categories containing vegetables and fruit and which of the student-centered reasons may influence PW. Design: A cross-sectional study (from December, 2017 to June, 2018) ; the aggregate selective plate waste method ; the taste-and-rate method (food preferences) ; the multiple-choice survey (student-centered reasons for the PW occurrence). Setting: Fourteen primary schools (Zagreb). Participants: Students aged 7–10 years ; 17, 163 meals (PW) ; 11, 960 meals (PW and food preferences for meals containing vegetables and fruit) ; 6, 507 meals (student-centered factors). Main Outcome Measures: Plate waste, preferences, and student-centered reasons for PW occurrence. Analysis: Continuous and categorical data were analyzed using a 1-way analysis of variance for nonnormal distribution with post hoc Dunnett's test and chi-square test of homogeneity, respectively. Spearman's rank correlation coefficient was performed to examine the correlation between the amount of PW and served food. All statistical analyses were constructed with robust standard errors clustered at the school level. Results: Among the different types of vegetables and fruit meals, students wasted lower amounts of fruit (10.3% ; P = 0.005). Plate waste was positively correlated with the amount of served food (r = 0.93 ; P < 0.001) containing vegetables and fruit. Students preferred fruit and starchy food with vegetables. Among personal factors for not finishing meals, the most frequent reason was they did not like the taste of the food. Conclusion and Implications: Findings highlight the importance of food preferences and other student-centered reasons in explaining PW by primary school students.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Croatian Scientific ...arrow_drop_down
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    Journal of Nutrition Education and Behavior
    Article . 2022 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Croatian Scientific ...arrow_drop_down
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      Journal of Nutrition Education and Behavior
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    Authors: Ana, Ilić; Ivana, Rumbak; Ružica, Brečić; Irena, Colić Barić; +1 Authors

    BackgroundSince the beneficial effects of fruit and vegetable (FV) consumption on health are well known due to the synergy of their nutrients and non-nutrients, it is crucial to have good tools to assess the FV intake. A food frequency questionnaire (FFQ) is an adequate method to estimate FV consumption, but it is necessary to relate this dietary method to the geographic and cultural environment. Therefore, this study presented the development of a semi-quantitative FFQ to estimate the FV intake in school-aged children who usually consume cooked homemade and school meals. It also aimed to evaluate the relative validity and reproducibility of the FFQ.MethodsSchool-aged children (baseline age 8 years) from 14 primary schools in the city of Zagreb participated in the study during the 2019/2020 school year. Parents/caregivers, together with the children, completed the FFQs and 3-day dietary records (3DDRs). The FFQ was designed to assess the consumption of eight food categories. The FFQ was validated using the 3DDR of 141 children (51.4% of boys), whereas the reproducibility test included the FFQ of 161 children (53.4% of boys).ResultsOf the eight food categories, FFQ overestimates the consumption of three and underestimates the consumption of three food categories (p &lt; 0.05; Wilcoxon signed rank test) compared to the 3DDR. De-attenuated correlation coefficients estimated a significant relationship (0.217–0.384) between the FFQ and 3DDR. Cross-classification analysis revealed that overall, 28–41% of children were classified in the same quartile, whereas less than 10% of children were extremely misclassified for all food categories obtained from 3DDR and FFQ1. κw values showed fair agreement for all food categories. The Bland–Altman analysis results showed a relatively small bias for all food categories (median between -11.7 and -54.8 g), with no systematic patterns between the FFQ and 3DDR. No differences were found between food categories estimated with the FFQs on both occasions, and Spearman’s correlation coefficients ranged from 0.664 to 0.712 (p &lt; 0.01). Cronbach’s alpha values (α &gt; 0.700) indicate good internal consistency, and ICCs (range 0.724–0.826; p &lt; 0.01) indicate good reproducibility of the FFQ.ConclusionThe results indicate reasonable relative validity and acceptable reproducibility of the FFQ for estimating FV consumption among school-aged children.

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    Frontiers in Nutrition
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    Authors: Ivana Martinčević; Predrag Brlek; Nives Domjan Kačarević;

    In order to meet the needs and requirements of visitors in tourist destinations related to modes of transport, the offer in tourist destinations must satisfy travel habits. The introduction of the MaaS (Mobility as a Service) concept is reflected in the reduction of dependence on private cars. The main objectives of this paper are (1) to explore the MaaS concept, awareness about the concept, its importance and benefits in general and related to tourism in the Republic of Croatia; and (2) to explore the connection between a multimodal mindset about mobility concepts on the very concept of MaaS under the influence of satisfaction using mobility-related applications in tourism. The results of the research confirm a statistically significant relationship between a multimodal mindset (way of thinking) and the MaaS service in the function of tourism, and that relationship is mediated by the satisfaction with the use of mobility-related applications, but also the lack of awareness about the concept itself.

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    Sustainability
    Other literature type . Article . 2022 . Peer-reviewed
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    Authors: Márcio A. G. Coelho; Gareth A. Pearson; Joana R. H. Boavida; Diogo Paulo; +13 Authors

    The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals This work was funded by program BIOMARES, a Pew Marine Fellowship, and FCT – Foundation for Science and Technology UIDB/04326/2020, UIDP/04326/2020, LA/P/0101/2020, PTDC/BIA-CBI/6515/2020; EU-BiodivERsA BiodivRestore-253 (FCT: DivRestore/0013/2020); EU Horizon 2020 research and innovation program (MERCES: Grant no. 689518); as well as the Inaqua Conservation Fund (Oceanário de Lisboa and National Geographic Channel) and National Geographic Society/Waitt Grant no. W153-11. This article is also a product of the French network on Marine Connectivity (GDR MarCo). M.C. was supported by postdoctoral fellowships of projects HABMAR (Grant no. MAR-01.04.02-FEAMP-0018) co-financed by the European Maritime and Fisheries Fund of the Operational Program MAR 2020 for Portugal (Portugal 2020), and BiodivAMP (Grant no. FA_06_2017_045) financed by the Directorate-General for Sea Policy of the Ministry of Economy and Sea for Portugal under the Operational Program Fundo Azul. JBL was funded by assistant researcher contract framework of the RD Unit—UID/Multi/04423/2019 – Interdisciplinary Centre of Marine and Environmental Research—financed by the European Regional Development Fund (ERDF) through COMPETE2020—Operational Program for Competitiveness and Internationalization (POCI) and national funds through FCT/MCTES (PIDDAC). JB was funded by a FCT PhD scholarship SFRH/BD/72501/2010 21 pages, 4 figures, 1 table, supporting information https://doi.org/10.1002/ece3.9740.-- Data Availability Statement: Raw RNA-seq data and mt-mutS sequences have been uploaded to NCBI's SRA (BioProject ID: PRJNA847883) and GenBank databases (Accessions numbers: ON804207-ON804214), respectively. Final sequence alignments and microsatellite data are available on DRYAD (https://doi.org/10.5061/dryad.r7sqv9sfm) With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) Peer reviewed

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    Authors: Ilić, Ana; Rumbak, Ivana; Marić, Lucija; Karlović, Tea; +3 Authors

    In countries around the world, a dietary shift is observed in which the consumption of highly processed foods increases over unprocessed or minimally processed foods. The objective of this study was to observe the proportion of processed foods in the diet of school-aged children and to assess how this relates to sex, weight status and school meal consumption. The aim was to assess the impact of processed foods on overall diet quality in terms of ultra-processed foods contribution to total daily energy intake. Dietary intake was observed from dietary records for three non-consecutive days of 168 children (50.6% boys) aged 8.3 ± 0.5 years. All foods and beverages were classified into four groups according to NOVA food classification system. The contribution of each NOVA food group to total daily energy intake was calculated and the mean nutrient intake of children divided into terciles according to total daily energy intake from ultra-processed foods was compared. Anthropometric measurements were performed according to standard protocols, while sex and age z-scores were obtained using AnthroPlus software. Results show that unprocessed or minimally processed foods (38.1% kcal) and ultra-processed foods (38.1% kcal) had the highest proportion of dietary intake. There was no difference in NOVA food groups intake by sex or weight status, while number of school meals may contribute to the intake of processed culinary ingredients. Children who had higher energy intake from ultra-processed foods had lower intake of animal proteins (p=0.009), polyunsaturated fatty acids (p=0.014), vitamin A (p=0.027) and most minerals, but higher intake of carbohydrates (p=0.014) and copper (p=0.014) compared to children with lower energy intake from ultra-processed foods. In conclusion, school-aged children had equal share of energy from unprocessed or minimally processed foods and from ultra-processed foods. Higher share of energy from ultra-process foods may contribute to poor overall nutrition.

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    Croatian Journal of Food Science and Technology
    Article . 2022 . Peer-reviewed
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      Croatian Journal of Food Science and Technology
      Article . 2022 . Peer-reviewed
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    Authors: Igor Živković; Jan Gačnik; Slaven Jozić; Jože Kotnik; +2 Authors

    Wet deposition is the main source of mercury (Hg) from the atmosphere to the Earth’s surface. However, the processes that govern the dispersion of deposited Hg in seawater are currently not well understood. To address this issue, total mercury (THg) concentrations in surface seawaters and precipitation were determined on a monthly basis in the Bay of Kaštela (Central Adriatic Sea). Following the assumption that deposited THg is diluted in the seawater bulk due to mixing processes, an exponential decay-like model was developed and the wet deposition of THg was normalized based on periods between precipitation events and seawater sampling. Normalized wet deposition of THg showed significant correlation with the THg gradient in surface seawater after removal of an outlier. To explain the observed outlier, further data normalization included wind data to account for enhanced seawater mixing due to strong winds. Wind-normalized THg deposition of all datapoints showed significant correlation with the THg gradient in surface seawater. The correlation showed that the THg gradient in surface seawater of 0.378 pg L−1 m−1 corresponds to THg wet deposition of 1 ng m−2 after including the influence of wind speed on seawater mixing.

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    Authors: Angela Tregear; Zorica Aničić; Filippo Arfini; Beatrice Biasini; +17 Authors

    Increasingly, policymakers are setting ambitious goals for sustainability in public procurement, integrated across different pillars. Such ambitions are apparent in public catering services, where procurement models have been shifting towards greater localisation of supply chains and purchasing of more organically grown food. To date however, few studies have examined empirically what the impacts of different procurement models are across these multiple pillars of sustainability. This research aimed to fill the gap, by measuring and comparing the environmental, economic and nutritional outcomes of different models of school meals procurement. Case studies were undertaken of ten primary school meals services in five European countries, capturing different procurement model types. Results showed carbon emissions ranged from 0.95 kgs CO2e per meal in the lowest case to 2.41 kgs CO2e in the highest case, with adoption of low carbon food waste disposal methods and reduction of the amount of ruminant meat in the menus being the most important actions for lowering emissions. In terms of economic impact, local economic multiplier ratios ranged from 1.59 to 2.46, and although the level of local food sourcing contributed to these ratios, the effect was eclipsed, in some cases, by investment in local catering staff. Meanwhile, implementation of a robust standards regime and improving canteen environment and supervision were the most important actions for nutritional quality and intake. The paper discusses the implications of the findings for integrated, sustainable models of food procurement.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Tatjana Bakran-Petricioli; Dajana Kujundžić; Martina Naranđa; Donat Petricioli; +2 Authors

    In the last few years, the endemic Mediterranean bivalve Pinna nobilis has been exposed to dramatic mortality in its entire area, which could lead to the extinction of the species. Throughout the Mediterranean, a lot of effort is being put into finding ways of preserving it. One of the methods used to monitor recruitment and juveniles’ survival is the installation of collectors for bivalve larvae. We installed collectors at two locations: in Brijuni National Park (North Adriatic) and Luka Cove (central Adriatic). Our aim was to compare the fouling community on the collectors in two consecutive years (2019 and 2020), especially because the installation of collectors in 2020 coincided with mass mortality events of P. nobilis in the area. The number and size of juvenile P. nobilis and the qualitative and quantitative composition of the fouling communities were determined. The results show a reduction in the number and size of juvenile P. nobilis and an explosion of the invasive bivalve Anadara transversa population on collectors in the second year. In Luka Cove, another invasive species—the ascidian Styela plicata—also seriously affected other organisms on the collectors to the point of preventing analysis of the fouling community.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Marine Sc...arrow_drop_down
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Márcio A. G. Coelho; Jean-Baptiste Ledoux; Joana Boavida; Diogo Paulo; +9 Authors

    The Gray's sea fan, Paramuricea grayi (Johnson, 1861), typically inhabits deep littoral and circalittoral habitats of the eastern temperate and tropical Atlantic Ocean. Along the Iberian Peninsula, where P. grayi is a dominant constituent of circalittoral coral gardens, two segregating lineages (yellow and purple morphotypes) were recently identified using single-copy nuclear orthologues. The mitochondrial genomes of 9 P. grayi individuals covering both color morphotypes were assembled from RNA-seq data, using samples collected at three sites in southern (Sagres and Tavira) and western (Cape Espichel) Portugal. The complete circular mitogenome is 18,668 bp in length, has an A + T-rich base composition (62.5%) and contains the 17 genes typically found in Octocorallia: 14 protein-coding genes (atp6, atp8, cob, cox1-3, mt-mutS, nad1-6, and nad4L), the small and large subunit rRNAs (rns and rnl), and one transfer RNA (trnM). The mitogenomes were nearly identical for all specimens, though we identified a noteworthy polymorphism (two SNPs 9 bp apart) in the mt-mutS of one purple individual that is shared with the sister species P. clavata. The mitogenomes of the two species have a pairwise sequence identity of 99.0%, with nad6 and mt-mutS having the highest rates of non-synonymous substitutions. info:eu-repo/semantics/publishedVersion MAR-01.04.02-FEAMP-0018 DivRestore/0013/2020 LA/P/0101/2020 FA_06_2017_045

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    Mitochondrial DNA Part B
    Article . 2022
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Mitochondrial DNA Pa...arrow_drop_down
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      Mitochondrial DNA Part B
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Ana Ilić; Ivana Rumbak; Ružica Brečić; Irena Colić Barić; +1 Authors

    Insufficient consumption of fruit and vegetables was found in primary school children. To address this problem, a three-year school-based multicomponent intervention was conducted in 14 primary schools in the City of Zagreb. The aim of the study was therefore to evaluate one of the primary goals of the intervention—the increase in fruit and vegetable intake among primary school children. A total of 681 children were allocated to the intervention (n = 300 in the control group and n = 381 in the intervention group). The intervention included 23 interactive classroom workshops, 10 cross-curricular activities, 13 homework challenges, visual exposure with educational posters in classrooms, parent education via the website, and the implementation of new dishes into the school food system. Fruit and vegetable intake was assessed using a semi-quantitative food frequency questionnaire at baseline and after the intervention. Data were analyzed by per-protocol analysis. The study involved 259 children (50.2% girls; age 7.7 ± 0.4 years; n = 116 in the control group and n = 143 in the intervention group) who completed a food intake frequency questionnaire at both time points. Children in the intervention group showed a significant increase (p < 0.001) in total daily fruit and vegetable intake (before: 332.1 ± 164.9 g; after: 430.1 ± 186.7 g) compared to the control group (before: 350.2 ± 187.5; after: 382.6 ± 196.8) after the intervention. The increase in fruit and vegetable intake was achieved in 89% of children, while 25% more children reached the daily recommendation of 400 g. The use of the multicomponent intervention showed potential to increase fruit and vegetable intake in primary school children.

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    Nutrients
    Article . 2022
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    Nutrients
    Article . 2022
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      Nutrients
      Article . 2022
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      Nutrients
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    Authors: Ana Ilić; Martina Bituh; Ružica Brečić; Irena Colić Barić;

    Objective: To assess differences in the amount of plate waste (PW) and food preferences among food categories containing vegetables and fruit and which of the student-centered reasons may influence PW. Design: A cross-sectional study (from December, 2017 to June, 2018) ; the aggregate selective plate waste method ; the taste-and-rate method (food preferences) ; the multiple-choice survey (student-centered reasons for the PW occurrence). Setting: Fourteen primary schools (Zagreb). Participants: Students aged 7–10 years ; 17, 163 meals (PW) ; 11, 960 meals (PW and food preferences for meals containing vegetables and fruit) ; 6, 507 meals (student-centered factors). Main Outcome Measures: Plate waste, preferences, and student-centered reasons for PW occurrence. Analysis: Continuous and categorical data were analyzed using a 1-way analysis of variance for nonnormal distribution with post hoc Dunnett's test and chi-square test of homogeneity, respectively. Spearman's rank correlation coefficient was performed to examine the correlation between the amount of PW and served food. All statistical analyses were constructed with robust standard errors clustered at the school level. Results: Among the different types of vegetables and fruit meals, students wasted lower amounts of fruit (10.3% ; P = 0.005). Plate waste was positively correlated with the amount of served food (r = 0.93 ; P < 0.001) containing vegetables and fruit. Students preferred fruit and starchy food with vegetables. Among personal factors for not finishing meals, the most frequent reason was they did not like the taste of the food. Conclusion and Implications: Findings highlight the importance of food preferences and other student-centered reasons in explaining PW by primary school students.

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    Journal of Nutrition Education and Behavior
    Article . 2022 . Peer-reviewed
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      Journal of Nutrition Education and Behavior
      Article . 2022 . Peer-reviewed
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    Authors: Ana, Ilić; Ivana, Rumbak; Ružica, Brečić; Irena, Colić Barić; +1 Authors

    BackgroundSince the beneficial effects of fruit and vegetable (FV) consumption on health are well known due to the synergy of their nutrients and non-nutrients, it is crucial to have good tools to assess the FV intake. A food frequency questionnaire (FFQ) is an adequate method to estimate FV consumption, but it is necessary to relate this dietary method to the geographic and cultural environment. Therefore, this study presented the development of a semi-quantitative FFQ to estimate the FV intake in school-aged children who usually consume cooked homemade and school meals. It also aimed to evaluate the relative validity and reproducibility of the FFQ.MethodsSchool-aged children (baseline age 8 years) from 14 primary schools in the city of Zagreb participated in the study during the 2019/2020 school year. Parents/caregivers, together with the children, completed the FFQs and 3-day dietary records (3DDRs). The FFQ was designed to assess the consumption of eight food categories. The FFQ was validated using the 3DDR of 141 children (51.4% of boys), whereas the reproducibility test included the FFQ of 161 children (53.4% of boys).ResultsOf the eight food categories, FFQ overestimates the consumption of three and underestimates the consumption of three food categories (p &lt; 0.05; Wilcoxon signed rank test) compared to the 3DDR. De-attenuated correlation coefficients estimated a significant relationship (0.217–0.384) between the FFQ and 3DDR. Cross-classification analysis revealed that overall, 28–41% of children were classified in the same quartile, whereas less than 10% of children were extremely misclassified for all food categories obtained from 3DDR and FFQ1. κw values showed fair agreement for all food categories. The Bland–Altman analysis results showed a relatively small bias for all food categories (median between -11.7 and -54.8 g), with no systematic patterns between the FFQ and 3DDR. No differences were found between food categories estimated with the FFQs on both occasions, and Spearman’s correlation coefficients ranged from 0.664 to 0.712 (p &lt; 0.01). Cronbach’s alpha values (α &gt; 0.700) indicate good internal consistency, and ICCs (range 0.724–0.826; p &lt; 0.01) indicate good reproducibility of the FFQ.ConclusionThe results indicate reasonable relative validity and acceptable reproducibility of the FFQ for estimating FV consumption among school-aged children.

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    Frontiers in Nutrition
    Article . 2022
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      Frontiers in Nutrition
      Article . 2022
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    Authors: Ivana Martinčević; Predrag Brlek; Nives Domjan Kačarević;

    In order to meet the needs and requirements of visitors in tourist destinations related to modes of transport, the offer in tourist destinations must satisfy travel habits. The introduction of the MaaS (Mobility as a Service) concept is reflected in the reduction of dependence on private cars. The main objectives of this paper are (1) to explore the MaaS concept, awareness about the concept, its importance and benefits in general and related to tourism in the Republic of Croatia; and (2) to explore the connection between a multimodal mindset about mobility concepts on the very concept of MaaS under the influence of satisfaction using mobility-related applications in tourism. The results of the research confirm a statistically significant relationship between a multimodal mindset (way of thinking) and the MaaS service in the function of tourism, and that relationship is mediated by the satisfaction with the use of mobility-related applications, but also the lack of awareness about the concept itself.

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    Sustainability
    Other literature type . Article . 2022 . Peer-reviewed
    License: CC BY
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    Article . 2022
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      Other literature type . Article . 2022 . Peer-reviewed
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    Authors: Márcio A. G. Coelho; Gareth A. Pearson; Joana R. H. Boavida; Diogo Paulo; +13 Authors

    The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals This work was funded by program BIOMARES, a Pew Marine Fellowship, and FCT – Foundation for Science and Technology UIDB/04326/2020, UIDP/04326/2020, LA/P/0101/2020, PTDC/BIA-CBI/6515/2020; EU-BiodivERsA BiodivRestore-253 (FCT: DivRestore/0013/2020); EU Horizon 2020 research and innovation program (MERCES: Grant no. 689518); as well as the Inaqua Conservation Fund (Oceanário de Lisboa and National Geographic Channel) and National Geographic Society/Waitt Grant no. W153-11. This article is also a product of the French network on Marine Connectivity (GDR MarCo). M.C. was supported by postdoctoral fellowships of projects HABMAR (Grant no. MAR-01.04.02-FEAMP-0018) co-financed by the European Maritime and Fisheries Fund of the Operational Program MAR 2020 for Portugal (Portugal 2020), and BiodivAMP (Grant no. FA_06_2017_045) financed by the Directorate-General for Sea Policy of the Ministry of Economy and Sea for Portugal under the Operational Program Fundo Azul. JBL was funded by assistant researcher contract framework of the RD Unit—UID/Multi/04423/2019 – Interdisciplinary Centre of Marine and Environmental Research—financed by the European Regional Development Fund (ERDF) through COMPETE2020—Operational Program for Competitiveness and Internationalization (POCI) and national funds through FCT/MCTES (PIDDAC). JB was funded by a FCT PhD scholarship SFRH/BD/72501/2010 21 pages, 4 figures, 1 table, supporting information https://doi.org/10.1002/ece3.9740.-- Data Availability Statement: Raw RNA-seq data and mt-mutS sequences have been uploaded to NCBI's SRA (BioProject ID: PRJNA847883) and GenBank databases (Accessions numbers: ON804207-ON804214), respectively. Final sequence alignments and microsatellite data are available on DRYAD (https://doi.org/10.5061/dryad.r7sqv9sfm) With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) Peer reviewed

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    Repositório do ISPA
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Repositório do ISPA
      Article . 2023
      License: CC BY
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Ilić, Ana; Rumbak, Ivana; Marić, Lucija; Karlović, Tea; +3 Authors

    In countries around the world, a dietary shift is observed in which the consumption of highly processed foods increases over unprocessed or minimally processed foods. The objective of this study was to observe the proportion of processed foods in the diet of school-aged children and to assess how this relates to sex, weight status and school meal consumption. The aim was to assess the impact of processed foods on overall diet quality in terms of ultra-processed foods contribution to total daily energy intake. Dietary intake was observed from dietary records for three non-consecutive days of 168 children (50.6% boys) aged 8.3 ± 0.5 years. All foods and beverages were classified into four groups according to NOVA food classification system. The contribution of each NOVA food group to total daily energy intake was calculated and the mean nutrient intake of children divided into terciles according to total daily energy intake from ultra-processed foods was compared. Anthropometric measurements were performed according to standard protocols, while sex and age z-scores were obtained using AnthroPlus software. Results show that unprocessed or minimally processed foods (38.1% kcal) and ultra-processed foods (38.1% kcal) had the highest proportion of dietary intake. There was no difference in NOVA food groups intake by sex or weight status, while number of school meals may contribute to the intake of processed culinary ingredients. Children who had higher energy intake from ultra-processed foods had lower intake of animal proteins (p=0.009), polyunsaturated fatty acids (p=0.014), vitamin A (p=0.027) and most minerals, but higher intake of carbohydrates (p=0.014) and copper (p=0.014) compared to children with lower energy intake from ultra-processed foods. In conclusion, school-aged children had equal share of energy from unprocessed or minimally processed foods and from ultra-processed foods. Higher share of energy from ultra-process foods may contribute to poor overall nutrition.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ HRČAK - Portal of sc...arrow_drop_down
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Croatian Journal of Food Science and Technology
    Article . 2022 . Peer-reviewed
    Data sources: Crossref
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ HRČAK - Portal of sc...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Croatian Journal of Food Science and Technology
      Article . 2022 . Peer-reviewed
      Data sources: Crossref
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    Authors: Igor Živković; Jan Gačnik; Slaven Jozić; Jože Kotnik; +2 Authors

    Wet deposition is the main source of mercury (Hg) from the atmosphere to the Earth’s surface. However, the processes that govern the dispersion of deposited Hg in seawater are currently not well understood. To address this issue, total mercury (THg) concentrations in surface seawaters and precipitation were determined on a monthly basis in the Bay of Kaštela (Central Adriatic Sea). Following the assumption that deposited THg is diluted in the seawater bulk due to mixing processes, an exponential decay-like model was developed and the wet deposition of THg was normalized based on periods between precipitation events and seawater sampling. Normalized wet deposition of THg showed significant correlation with the THg gradient in surface seawater after removal of an outlier. To explain the observed outlier, further data normalization included wind data to account for enhanced seawater mixing due to strong winds. Wind-normalized THg deposition of all datapoints showed significant correlation with the THg gradient in surface seawater. The correlation showed that the THg gradient in surface seawater of 0.378 pg L−1 m−1 corresponds to THg wet deposition of 1 ng m−2 after including the influence of wind speed on seawater mixing.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Marine Sc...arrow_drop_down
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Authors: Angela Tregear; Zorica Aničić; Filippo Arfini; Beatrice Biasini; +17 Authors

    Increasingly, policymakers are setting ambitious goals for sustainability in public procurement, integrated across different pillars. Such ambitions are apparent in public catering services, where procurement models have been shifting towards greater localisation of supply chains and purchasing of more organically grown food. To date however, few studies have examined empirically what the impacts of different procurement models are across these multiple pillars of sustainability. This research aimed to fill the gap, by measuring and comparing the environmental, economic and nutritional outcomes of different models of school meals procurement. Case studies were undertaken of ten primary school meals services in five European countries, capturing different procurement model types. Results showed carbon emissions ranged from 0.95 kgs CO2e per meal in the lowest case to 2.41 kgs CO2e in the highest case, with adoption of low carbon food waste disposal methods and reduction of the amount of ruminant meat in the menus being the most important actions for lowering emissions. In terms of economic impact, local economic multiplier ratios ranged from 1.59 to 2.46, and although the level of local food sourcing contributed to these ratios, the effect was eclipsed, in some cases, by investment in local catering staff. Meanwhile, implementation of a robust standards regime and improving canteen environment and supervision were the most important actions for nutritional quality and intake. The paper discusses the implications of the findings for integrated, sustainable models of food procurement.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Cleaner P...arrow_drop_down
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Journal of Cleaner P...arrow_drop_down
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