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Voltage-gated K+-channels modulatio...

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Genome mining and characterisation of a novel transaminase with remote stereoselectivity
descriptionPublicationkeyboard_double_arrow_right Article 2019 Ireland, Austria Publisher:Springer Science and Business Media LLCPublicly fundedFunded by:FWF | Voltage-gated K+-channels..., SFI | Pre-clinical testing of n..., IRC +2 projects
FWF| Voltage-gated K+-channels modulation by Ru-complexes ,
SFI| Pre-clinical testing of novel fungal biofilm blockers for the medical device sector. ,
IRC ,
SFI| Synthesis and Solid State Pharmaceutical Centre (SSPC) ,
EC| INMARE
Authors: Declan P. Gavin; F. J. Reen; Javier Rocha-Martín; I. Abreu-Castilla; +7 Authors
Declan P. Gavin; F. J. Reen; Javier Rocha-Martín; I. Abreu-Castilla; David Woods; Aoife Foley; Pedro A. Sánchez-Murcia; M. Schwarz; Pat O'Neill; Anita R. Maguire; Fergal O'Gara;
doi: 10.1038/s41598-019-56612-7
handle: 11353/10.1176402 , 10468/12604
pmid: 35624309 , 31889089
pmc: PMC6937235
doi: 10.1038/s41598-019-56612-7
handle: 11353/10.1176402 , 10468/12604
pmid: 35624309 , 31889089
pmc: PMC6937235
AbstractMicrobial enzymes from pristine niches can potentially deliver disruptive opportunities in synthetic routes to Active Pharmaceutical Ingredients and intermediates in the Pharmaceutical Industry. Advances in green chemistry technologies and the importance of stereochemical control, further underscores the application of enzyme-based solutions in chemical synthesis. The rich tapestry of microbial diversity in the oceanic ecosystem encodes a capacity for novel biotransformations arising from the chemical complexity of this largely unexplored bioactive reservoir. Here we report a novel ω-transaminase discovered in a marine sponge Pseudovibrio sp. isolate. Remote stereoselection using a transaminase has been demonstrated for the first time using this novel protein. Application to the resolution of an intermediate in the synthesis of sertraline highlights the synthetic potential of this novel biocatalyst discovered through genomic mining. Integrated chemico-genomics revealed a unique substrate profile, while molecular modelling provided structural insights into this ‘first in class’ selectivity at a remote chiral centre.
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Article . 2019
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Article . 2019 . Peer-reviewed
License: CC BY
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Cork Open Research Archive (CORA)
Article . 2019
License: CC BY
Data sources: Cork Open Research Archive (CORA)
Scientific Reports
Article . 2019
Data sources: DOAJ-Articles
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Article . 2019
Data sources: Europe PubMed Central
Cork Open Research Archive (CORA)
Article . 2019
Data sources: Cork Open Research Archive (CORA)
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Article . 2019
Full-Text: http://europepmc.org/articles/PMC6937235
Data sources: PubMed Central
Scientific Reports; Permanent Hosting, Archiving and Indexing of Digital Resources and Assets
Article . 2019 . Peer-reviewed
License: CC BY
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Scientific Reports
Article
License: CC BY
Data sources: UnpayWall
Cork Open Research Archive (CORA)
Article . 2019
License: CC BY
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Scientific Reports
Article . 2019
Data sources: DOAJ-Articles
Scientific Reports
Article . 2019
Data sources: Europe PubMed Central
Cork Open Research Archive (CORA)
Article . 2019
Data sources: Cork Open Research Archive (CORA)
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The following results are related to SDSN - Greece. Are you interested to view more results? Visit OpenAIRE - Explore.

Genome mining and characterisation of a novel transaminase with remote stereoselectivity
descriptionPublicationkeyboard_double_arrow_right Article 2019 Ireland, Austria Publisher:Springer Science and Business Media LLCPublicly fundedFunded by:FWF | Voltage-gated K+-channels..., SFI | Pre-clinical testing of n..., IRC +2 projects
FWF| Voltage-gated K+-channels modulation by Ru-complexes ,
SFI| Pre-clinical testing of novel fungal biofilm blockers for the medical device sector. ,
IRC ,
SFI| Synthesis and Solid State Pharmaceutical Centre (SSPC) ,
EC| INMARE
Authors: Declan P. Gavin; F. J. Reen; Javier Rocha-Martín; I. Abreu-Castilla; +7 Authors
Declan P. Gavin; F. J. Reen; Javier Rocha-Martín; I. Abreu-Castilla; David Woods; Aoife Foley; Pedro A. Sánchez-Murcia; M. Schwarz; Pat O'Neill; Anita R. Maguire; Fergal O'Gara;
doi: 10.1038/s41598-019-56612-7
handle: 11353/10.1176402 , 10468/12604
pmid: 35624309 , 31889089
pmc: PMC6937235
doi: 10.1038/s41598-019-56612-7
handle: 11353/10.1176402 , 10468/12604
pmid: 35624309 , 31889089
pmc: PMC6937235
AbstractMicrobial enzymes from pristine niches can potentially deliver disruptive opportunities in synthetic routes to Active Pharmaceutical Ingredients and intermediates in the Pharmaceutical Industry. Advances in green chemistry technologies and the importance of stereochemical control, further underscores the application of enzyme-based solutions in chemical synthesis. The rich tapestry of microbial diversity in the oceanic ecosystem encodes a capacity for novel biotransformations arising from the chemical complexity of this largely unexplored bioactive reservoir. Here we report a novel ω-transaminase discovered in a marine sponge Pseudovibrio sp. isolate. Remote stereoselection using a transaminase has been demonstrated for the first time using this novel protein. Application to the resolution of an intermediate in the synthesis of sertraline highlights the synthetic potential of this novel biocatalyst discovered through genomic mining. Integrated chemico-genomics revealed a unique substrate profile, while molecular modelling provided structural insights into this ‘first in class’ selectivity at a remote chiral centre.
downloaddonwloadFull-Text
Scientific Reportsarrow_drop_down
Scientific Reports
Article . 2019
Full-Text: http://europepmc.org/articles/PMC6937235
Data sources: PubMed Central
Scientific Reports; Permanent Hosting, Archiving and Indexing of Digital Resources and Assets
Article . 2019 . Peer-reviewed
License: CC BY
Data sources: Crossref; Permanent Hosting, Archiving and Indexing of Digital Resources and Assets
Scientific Reports
Article
License: CC BY
Data sources: UnpayWall
Cork Open Research Archive (CORA)
Article . 2019
License: CC BY
Data sources: Cork Open Research Archive (CORA)
Scientific Reports
Article . 2019
Data sources: DOAJ-Articles
Scientific Reports
Article . 2019
Data sources: Europe PubMed Central
Cork Open Research Archive (CORA)
Article . 2019
Data sources: Cork Open Research Archive (CORA)
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more_vert
downloaddonwloadFull-Text
Scientific Reportsarrow_drop_down
Scientific Reports
Article . 2019
Full-Text: http://europepmc.org/articles/PMC6937235
Data sources: PubMed Central
Scientific Reports; Permanent Hosting, Archiving and Indexing of Digital Resources and Assets
Article . 2019 . Peer-reviewed
License: CC BY
Data sources: Crossref; Permanent Hosting, Archiving and Indexing of Digital Resources and Assets
Scientific Reports
Article
License: CC BY
Data sources: UnpayWall
Cork Open Research Archive (CORA)
Article . 2019
License: CC BY
Data sources: Cork Open Research Archive (CORA)
Scientific Reports
Article . 2019
Data sources: DOAJ-Articles
Scientific Reports
Article . 2019
Data sources: Europe PubMed Central
Cork Open Research Archive (CORA)
Article . 2019
Data sources: Cork Open Research Archive (CORA)
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
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Voltage-gated K+-channels modulatio...

Genome mining and characterisation of a novel transaminase with remote stereoselectivity

Genome mining and characterisation of a novel transaminase with remote stereoselectivity

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Genome mining and characterisation of a novel transaminase with remote stereoselectivity

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Genome mining and characterisation of a novel transaminase with remote stereoselectivity

SDSN - Greece
Publications
Austrian Science Fund (FWF)
Voltage-gated K+-channels modulatio...