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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Hölscher, Katharina; Frantzeskaki, Niki; Kindlon, Donnchadh; Collier, Marcus; +8 Authors

    This paper seeks to understand how co-production can become embedded as a collaborative governance practice by which city governments plan, deliver and steward nature-based solutions. To these ends, the paper analyses how policy officers manifest capacities for co-production in three European cities – Genk (Belgium), Glasgow (United Kingdom), and Poznań (Poland) – while experimenting with co-production to develop and scale nature-based solutions. Co-production capacities include conditions and activities to (1) create space for co-production, (2) safeguard inclusive and legitimate co-production, and (3) link co-production processes and results to con- texts. The results demonstrate how policy officers in the three cities have mobilised and created resources, skills, institutional support and partnerships to implement diverse processes to co-produce nature-based solutions. While these conditions mark starting changes in urban governance, engaging with and embedding co-production causes tensions between the dynamic and diffuse nature of co-production and existing formal governance settings and processes. Lessons for strengthening the capacities to embed co-production as a collaborative governance practice in nature-based solutions planning, delivery, and stewardship are: (1) embedding a tailor-made approach for inclusive co-production to meaningfully engage diverse actors in place-based settings, (2) embedding open-ended co-production with long-term benefits, and (3) embedding new relations and roles to sustain co- production.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODO; Environmenta...arrow_drop_down
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    ZENODO; Environmental Science & Policy
    Article . 2024 . Peer-reviewed
    License: CC BY NC ND
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODO; Environmenta...arrow_drop_down
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      ZENODO; Environmental Science & Policy
      Article . 2024 . Peer-reviewed
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Marcus J. Collier; Niki Frantzeskaki; Stuart Connop; Gillian Dick; +19 Authors

    Mainstreaming nature-based solutions in cities has grown in scale and magnitude in recent times but is still considered to be the main challenge for transitioning our cities and their communities to be more climate resilient and liveable: environmentally, economically, and socially. Furthermore, taking nature-based solutions to the next level, and scaling them out to all urban contexts to achieve a greater impact, is proving to be slow and often conflicts with other transitioning initiatives such as energy generation, mobility and transport initiatives, and infilling to combat sprawl. So, the task is neither easy nor straightforward; there are many barriers to this novel transition, especially when it comes to collaborative approaches to implementing nature-based solutions with diverse urban communities and within city authorities themselves. This paper reports on a new process that is systematically co-produced and captured as a framework for planning nature-based solutions that emerged during the Connecting Nature project. The Connecting Nature Framework is a three-stage, iterative process that involves seven key activity areas for mainstreaming nature-based solutions: technical solutions, governance, financing and business models, nature-based enterprises, co-production, reflexive monitoring, and impact assessment. The tested and applied framework is designed to address and overcome barriers to the implementation of nature-based solutions in cities via a co-created, iterative, and reflective approach. The planning process guided by the proposed framework has already yielded promising results with some of the cities of the project, though further usage and its adoption by other cities is needed to explore its potential in different contexts, especially in the Global South. The paper concludes with suggestions on how this may be realised.

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    Nature-Based Solutions
    Article . 2023
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    ZENODO; Nature-Based Solutions
    Article . 2023 . Peer-reviewed
    License: CC BY NC ND
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      Nature-Based Solutions
      Article . 2023
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      ZENODO; Nature-Based Solutions
      Article . 2023 . Peer-reviewed
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Hölscher, Katharina; Frantzeskaki, Niki; Collier, Marcus; Connop, Stuart; +26 Authors

    PUBLISHED This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding of how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver, and steward NBS. Based on these activities of the policy officers, the analysis identifies three strategies, associated stepping stones and changes in governance conditions, to mainstream NBS in governance capacities: institutionalising (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements. This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding about how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver and steward NBS. The analysis identifies three mainstreaming strategies to develop capacities for (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Trinity's Access to ...arrow_drop_down
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    npj Urban Sustainability
    Article . 2023
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    ZENODO; npj Urban Sustainability
    Article . 2023 . Peer-reviewed
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      npj Urban Sustainability
      Article . 2023
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      Article . 2023 . Peer-reviewed
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    Authors: Smith P.; Le Devendec L.; Jouy E.; Larvor E.; +26 Authors

    This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested applying standard methods specifying incubation at 28 °C for 24-28h. Aggregated MIC distributions for a total of 247 isolates were determined in nine laboratories for eleven agents. Data aggregations of the disc zone for the ten agents analysed contained between 157 and 218 observations made by four to seven laboratories. Acceptable ranges for quality control (QC) reference strains were available for seven agents and the related multi-laboratory aggregated data were censored excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg/ml), gentamicin (≤4 µg/ml), oxytetracycline (≤0.25 µg/ml) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg/ml). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available or not, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole) and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to CLSI for their consideration in setting international consensus epidemiological cut-off values. International audience

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    Research@WUR
    Article . 2023
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    Authors: Pierluigi Penna; Filippo Domenichetti; Andrea Belardinelli; Michela Martinelli;

    In the last decade, an enormous amount of georeferenced oceanographic data, co-located with catch information, have been collected through the use of commercial fishing vessels operating in the Adriatic Sea and belonging to the fleet monitored by the AdriFOOS (Adriatic Fishery and Oceanography Observing System) infrastructure. This document describes the dataset of depth (pressure) and temperature profiles collected by means of AdriFOOS in the period 2012–2020 (available at https://doi.org/10.17882/73008, Penna et al., 2020) and briefly illustrates the structure of this facility, the oceanographic dataset validation procedures, and some of the results achieved, and it also lists some possible operational applications. This information is of the utmost importance to provide data to feed oceanographic models and advance knowledge on climate change, as well as to improve the ecosystem approach to fishery management.

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    Earth System Science Data (ESSD); CNR ExploRA
    Article . 2023 . Peer-reviewed
    License: CC BY
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    Earth System Science Data
    Article . 2023
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      Earth System Science Data (ESSD); CNR ExploRA
      Article . 2023 . Peer-reviewed
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      Earth System Science Data
      Article . 2023
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    Authors: Smith P.; Cortinovis L.; Pretto T.; Manfrin A.; +10 Authors

    The lack of internationally harmonised criteria for interpreting the data generated by standardised susceptibility testing methods presents a serious obstacle for the development of prudent use of antimicrobials in aquaculture. The data required to set epidemiological cut-off values for minimum inhibitory concentrations for antibiotic agents against Vibrio harveyi was determined using a standard microdilution method that specified the use of cation-adjusted Mueller Hinton broth and incubation at 28°C for 24 to 28 h. In total, 120 observations were made in 4 independent laboratories from 109 unique isolates. The aggregated data from these laboratories were analysed by the normalised resistance method and by ECOFFinder to calculate epidemiological cut-off values. The data for chloramphenicol, meropenem and sulfamethoxazole were not considered as suitable for analysis. The data for ampicillin indicated that this species is innately resistant to this agent. No acceptable ranges for quality control strains have been set for ceftazidime and, therefore, only provisional cut-off values could be generated for this agent. The epidemiological cut-off values were, however, calculated for the other 6 agents. These values were ≤0.5 µg ml-1 for enrofloxacin, ≤1 µg ml-1 for florfenicol, oxolinic acid and oxytetracycline, ≤4 µg ml-1 for gentamicin and ≤0.5/9.5 µg ml-1 for trimethoprim/sulfamethoxazole. Evidence is presented demonstrating that the data for these 6 antimicrobial agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for V. harveyi.

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    Authors: Miguel‐Berges, María L.; Mouratidou, Theodora; Santaliestra‐Pasias, Alba; Androutsos, Odysseas; +9 Authors

    SummaryBackgroundLifestyle behaviours related to diet and physical activity are associated with increased risk of obesity and evidence suggests that associations might be stronger when a synergetic effect is examined.ObjectiveTo examine the cross‐sectional and longitudinal associations between diet, screen time (ST) and step recommendations and risk of overweight and obesity in European preschoolers participating in the ToyBox‐study.MethodsIn this cluster‐randomized clinical trial, 718 children (51.4% boys) from six European countries participated. Parents filled out questionnaires with information on socio‐demographic status, step recommendations and ST.ResultsLongitudinal results indicate that participants having a low Diet Quality Index (DQI), not meeting ST and step recommendations at T0 and T1 had higher odds of having overweight/obesity at T1 (odds ratio [OR] = 1.116; 95% confidence interval [CI] = 1.104–2.562) than those children having a high DQI and meeting ST and step recommendations at T0 and T1. Similarly, participants having a high DQI, but not meeting ST and step recommendations at T0 and T1 had increased odds of having overweight/obesity (OR = 2.515; 95% CI = 1.171–3.021).ConclusionsThe proportion of participants having a low DQI, not adhering to both step and ST recommendations was very high, and it was associated with a higher probability of having overweight and obesity.

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    Pediatric Obesity
    Article . 2023 . Peer-reviewed
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      Pediatric Obesity
      Article . 2023 . Peer-reviewed
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Jose A. Caparrós-Martín; Montserrat Saladie; S. Patricia Agudelo-Romero; F. Jerry Reen; +5 Authors

    Abstract Background Cystic Fibrosis (CF) is a genetic condition characterized by neutrophilic inflammation and recurrent infection of the airways. How these processes are initiated and perpetuated in CF remains largely unknown. We have demonstrated a link between the intestinal microbiota-related metabolites bile acids (BA) and inflammation in the bronchoalveolar lavage fluid (BALF) from children with stable CF lung disease. To establish if BA indicate early pathological processes in CF lung disease, we combined targeted mass spectrometry and amplicon sequencing-based microbial characterization of 121 BALF specimens collected from 12-month old infants with CF enrolled in the COMBAT-CF study, a multicentre randomized placebo-controlled clinical trial comparing azithromycin versus placebo. We evaluated whether detection of BA in BALF is associated with the establishment of the inflammatory and microbial landscape of early CF lung disease, and whether azithromycin, a motilin agonist that has been demonstrated to reduce aspiration of gastric contents, alters the odds of detecting BA in BALF. We also explored how different prophylactic antibiotics regimens impact the early life BALF microbiota. Results Detection of BA in BALF was strongly associated with biomarkers of airway inflammation, more exacerbation episodes during the first year of life, increased use of oral antibiotics with prolonged treatment periods, a higher degree of structural lung damage, and distinct microbial profiles. Treatment with azithromycin, a motilin agonist, which has been reported to reduce aspiration of gastric contents, did not reduce the odds of detecting BA in BALF. Culture and molecular methods showed that azithromycin does not alter bacterial load or diversity in BALF. Conversely, penicillin-type prophylaxis reduced the odds of detecting BAs in BALF, which was associated with elevated levels of circulating biomarkers of cholestasis. We also observed that environmental factors such as penicillin-type prophylaxis or BAs detection were linked to distinct early microbial communities of the CF airways, which were associated with different inflammatory landscapes but not with structural lung damage. Conclusions Detection of BA in BALF portend early pathological events in CF lung disease. Benefits early in life associated with azithromycin are not linked to its antimicrobial properties.

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    Microbiome
    Article . 2023
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    Microbiome
    Article . 2022
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    Microbiome
    Article . 2023 . Peer-reviewed
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      Microbiome
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      Microbiome
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  • Authors: Jose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; +6 Authors

    AbstractBackgroundChronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in COPD patients and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage fluid (BAL) as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes, and bacterial lung colonisation.MethodsWe used BAL specimens from a cohort of COPD patients and healthy controls. Bile acids were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL cell pellets and quantified using qPCR. We profiled the BAL microbiota using an amplicon sequencing approach targeting the V3-V4 region of the 16S rRNA gene.ResultsDetection of BAs in BAL was more likely at earliest clinical stages of COPD and was independent of the degree of airway obstruction. BAL specimens with BAs demonstrated higher bacterial biomass and lower diversity. Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. Detection of BAs in BAL was not associated with either inflammatory markers or clinical outcomes. We also observed different bacterial community types in BAL, which were associated with different clinical groups, levels of inflammatory markers, and the degree of airway obstruction.ConclusionDetection of BAs in BAL was associated with different parameters of airway ecology. Further studies are needed to evaluate whether BAs in BAL can be used to stratify patients and for predicting disease progression trajectories.

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    Authors: Katharina Hölscher; Niki Frantzeskaki; Marcus Collier; Stuart Connop; +26 Authors

    Abstract This paper identifies and examines the strategies and governance conditions by which ten European cities have started mainstreaming nature-based solutions (NBS) as innovative systemic solutions in urban planning. The three sets of mainstreaming strategies focus on how teams of policy entrepreneurs within the cities experimented with innovative governance processes and mechanisms to strategically develop procedures, knowledge, skills, and relationships to integrate (a) a systems’ approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised interventions, and (c) reflexivity and learning about how they interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies demonstrate how starting from NBS as a type of systemic innovation can help promote shifts in urban planning to address complex sustainability problems in cities, and how starting points and specific processes depend on a respective city’s contexts and needs.

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    https://doi.org/10.21203/rs.3....
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      https://doi.org/10.21203/rs.3....
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Hölscher, Katharina; Frantzeskaki, Niki; Kindlon, Donnchadh; Collier, Marcus; +8 Authors

    This paper seeks to understand how co-production can become embedded as a collaborative governance practice by which city governments plan, deliver and steward nature-based solutions. To these ends, the paper analyses how policy officers manifest capacities for co-production in three European cities – Genk (Belgium), Glasgow (United Kingdom), and Poznań (Poland) – while experimenting with co-production to develop and scale nature-based solutions. Co-production capacities include conditions and activities to (1) create space for co-production, (2) safeguard inclusive and legitimate co-production, and (3) link co-production processes and results to con- texts. The results demonstrate how policy officers in the three cities have mobilised and created resources, skills, institutional support and partnerships to implement diverse processes to co-produce nature-based solutions. While these conditions mark starting changes in urban governance, engaging with and embedding co-production causes tensions between the dynamic and diffuse nature of co-production and existing formal governance settings and processes. Lessons for strengthening the capacities to embed co-production as a collaborative governance practice in nature-based solutions planning, delivery, and stewardship are: (1) embedding a tailor-made approach for inclusive co-production to meaningfully engage diverse actors in place-based settings, (2) embedding open-ended co-production with long-term benefits, and (3) embedding new relations and roles to sustain co- production.

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    ZENODO; Environmental Science & Policy
    Article . 2024 . Peer-reviewed
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    Authors: Marcus J. Collier; Niki Frantzeskaki; Stuart Connop; Gillian Dick; +19 Authors

    Mainstreaming nature-based solutions in cities has grown in scale and magnitude in recent times but is still considered to be the main challenge for transitioning our cities and their communities to be more climate resilient and liveable: environmentally, economically, and socially. Furthermore, taking nature-based solutions to the next level, and scaling them out to all urban contexts to achieve a greater impact, is proving to be slow and often conflicts with other transitioning initiatives such as energy generation, mobility and transport initiatives, and infilling to combat sprawl. So, the task is neither easy nor straightforward; there are many barriers to this novel transition, especially when it comes to collaborative approaches to implementing nature-based solutions with diverse urban communities and within city authorities themselves. This paper reports on a new process that is systematically co-produced and captured as a framework for planning nature-based solutions that emerged during the Connecting Nature project. The Connecting Nature Framework is a three-stage, iterative process that involves seven key activity areas for mainstreaming nature-based solutions: technical solutions, governance, financing and business models, nature-based enterprises, co-production, reflexive monitoring, and impact assessment. The tested and applied framework is designed to address and overcome barriers to the implementation of nature-based solutions in cities via a co-created, iterative, and reflective approach. The planning process guided by the proposed framework has already yielded promising results with some of the cities of the project, though further usage and its adoption by other cities is needed to explore its potential in different contexts, especially in the Global South. The paper concludes with suggestions on how this may be realised.

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    Nature-Based Solutions
    Article . 2023
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    ZENODO; Nature-Based Solutions
    Article . 2023 . Peer-reviewed
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      Nature-Based Solutions
      Article . 2023
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      ZENODO; Nature-Based Solutions
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    Authors: Hölscher, Katharina; Frantzeskaki, Niki; Collier, Marcus; Connop, Stuart; +26 Authors

    PUBLISHED This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding of how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver, and steward NBS. Based on these activities of the policy officers, the analysis identifies three strategies, associated stepping stones and changes in governance conditions, to mainstream NBS in governance capacities: institutionalising (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements. This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding about how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver and steward NBS. The analysis identifies three mainstreaming strategies to develop capacities for (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements.

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    npj Urban Sustainability
    Article . 2023
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    ZENODO; npj Urban Sustainability
    Article . 2023 . Peer-reviewed
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      npj Urban Sustainability
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    Authors: Smith P.; Le Devendec L.; Jouy E.; Larvor E.; +26 Authors

    This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested applying standard methods specifying incubation at 28 °C for 24-28h. Aggregated MIC distributions for a total of 247 isolates were determined in nine laboratories for eleven agents. Data aggregations of the disc zone for the ten agents analysed contained between 157 and 218 observations made by four to seven laboratories. Acceptable ranges for quality control (QC) reference strains were available for seven agents and the related multi-laboratory aggregated data were censored excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg/ml), gentamicin (≤4 µg/ml), oxytetracycline (≤0.25 µg/ml) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg/ml). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available or not, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole) and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to CLSI for their consideration in setting international consensus epidemiological cut-off values. International audience

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    Research@WUR
    Article . 2023
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    Brage IMR
    Article . 2023
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    Authors: Pierluigi Penna; Filippo Domenichetti; Andrea Belardinelli; Michela Martinelli;

    In the last decade, an enormous amount of georeferenced oceanographic data, co-located with catch information, have been collected through the use of commercial fishing vessels operating in the Adriatic Sea and belonging to the fleet monitored by the AdriFOOS (Adriatic Fishery and Oceanography Observing System) infrastructure. This document describes the dataset of depth (pressure) and temperature profiles collected by means of AdriFOOS in the period 2012–2020 (available at https://doi.org/10.17882/73008, Penna et al., 2020) and briefly illustrates the structure of this facility, the oceanographic dataset validation procedures, and some of the results achieved, and it also lists some possible operational applications. This information is of the utmost importance to provide data to feed oceanographic models and advance knowledge on climate change, as well as to improve the ecosystem approach to fishery management.

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    Earth System Science Data (ESSD); CNR ExploRA
    Article . 2023 . Peer-reviewed
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    Earth System Science Data
    Article . 2023
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    Authors: Smith P.; Cortinovis L.; Pretto T.; Manfrin A.; +10 Authors

    The lack of internationally harmonised criteria for interpreting the data generated by standardised susceptibility testing methods presents a serious obstacle for the development of prudent use of antimicrobials in aquaculture. The data required to set epidemiological cut-off values for minimum inhibitory concentrations for antibiotic agents against Vibrio harveyi was determined using a standard microdilution method that specified the use of cation-adjusted Mueller Hinton broth and incubation at 28°C for 24 to 28 h. In total, 120 observations were made in 4 independent laboratories from 109 unique isolates. The aggregated data from these laboratories were analysed by the normalised resistance method and by ECOFFinder to calculate epidemiological cut-off values. The data for chloramphenicol, meropenem and sulfamethoxazole were not considered as suitable for analysis. The data for ampicillin indicated that this species is innately resistant to this agent. No acceptable ranges for quality control strains have been set for ceftazidime and, therefore, only provisional cut-off values could be generated for this agent. The epidemiological cut-off values were, however, calculated for the other 6 agents. These values were ≤0.5 µg ml-1 for enrofloxacin, ≤1 µg ml-1 for florfenicol, oxolinic acid and oxytetracycline, ≤4 µg ml-1 for gentamicin and ≤0.5/9.5 µg ml-1 for trimethoprim/sulfamethoxazole. Evidence is presented demonstrating that the data for these 6 antimicrobial agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for V. harveyi.

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    Authors: Miguel‐Berges, María L.; Mouratidou, Theodora; Santaliestra‐Pasias, Alba; Androutsos, Odysseas; +9 Authors

    SummaryBackgroundLifestyle behaviours related to diet and physical activity are associated with increased risk of obesity and evidence suggests that associations might be stronger when a synergetic effect is examined.ObjectiveTo examine the cross‐sectional and longitudinal associations between diet, screen time (ST) and step recommendations and risk of overweight and obesity in European preschoolers participating in the ToyBox‐study.MethodsIn this cluster‐randomized clinical trial, 718 children (51.4% boys) from six European countries participated. Parents filled out questionnaires with information on socio‐demographic status, step recommendations and ST.ResultsLongitudinal results indicate that participants having a low Diet Quality Index (DQI), not meeting ST and step recommendations at T0 and T1 had higher odds of having overweight/obesity at T1 (odds ratio [OR] = 1.116; 95% confidence interval [CI] = 1.104–2.562) than those children having a high DQI and meeting ST and step recommendations at T0 and T1. Similarly, participants having a high DQI, but not meeting ST and step recommendations at T0 and T1 had increased odds of having overweight/obesity (OR = 2.515; 95% CI = 1.171–3.021).ConclusionsThe proportion of participants having a low DQI, not adhering to both step and ST recommendations was very high, and it was associated with a higher probability of having overweight and obesity.

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    Pediatric Obesity
    Article . 2023 . Peer-reviewed
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      Pediatric Obesity
      Article . 2023 . Peer-reviewed
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    Authors: Jose A. Caparrós-Martín; Montserrat Saladie; S. Patricia Agudelo-Romero; F. Jerry Reen; +5 Authors

    Abstract Background Cystic Fibrosis (CF) is a genetic condition characterized by neutrophilic inflammation and recurrent infection of the airways. How these processes are initiated and perpetuated in CF remains largely unknown. We have demonstrated a link between the intestinal microbiota-related metabolites bile acids (BA) and inflammation in the bronchoalveolar lavage fluid (BALF) from children with stable CF lung disease. To establish if BA indicate early pathological processes in CF lung disease, we combined targeted mass spectrometry and amplicon sequencing-based microbial characterization of 121 BALF specimens collected from 12-month old infants with CF enrolled in the COMBAT-CF study, a multicentre randomized placebo-controlled clinical trial comparing azithromycin versus placebo. We evaluated whether detection of BA in BALF is associated with the establishment of the inflammatory and microbial landscape of early CF lung disease, and whether azithromycin, a motilin agonist that has been demonstrated to reduce aspiration of gastric contents, alters the odds of detecting BA in BALF. We also explored how different prophylactic antibiotics regimens impact the early life BALF microbiota. Results Detection of BA in BALF was strongly associated with biomarkers of airway inflammation, more exacerbation episodes during the first year of life, increased use of oral antibiotics with prolonged treatment periods, a higher degree of structural lung damage, and distinct microbial profiles. Treatment with azithromycin, a motilin agonist, which has been reported to reduce aspiration of gastric contents, did not reduce the odds of detecting BA in BALF. Culture and molecular methods showed that azithromycin does not alter bacterial load or diversity in BALF. Conversely, penicillin-type prophylaxis reduced the odds of detecting BAs in BALF, which was associated with elevated levels of circulating biomarkers of cholestasis. We also observed that environmental factors such as penicillin-type prophylaxis or BAs detection were linked to distinct early microbial communities of the CF airways, which were associated with different inflammatory landscapes but not with structural lung damage. Conclusions Detection of BA in BALF portend early pathological events in CF lung disease. Benefits early in life associated with azithromycin are not linked to its antimicrobial properties.

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    Microbiome
    Article . 2023
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    Microbiome
    Article . 2022
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    Microbiome
    Article . 2023 . Peer-reviewed
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      Microbiome
      Article . 2022
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      Microbiome
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  • Authors: Jose A. Caparrós-Martín; Montserrat Saladié; S. Patricia Agudelo-Romero; Kristy S. Nichol; +6 Authors

    AbstractBackgroundChronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in COPD patients and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage fluid (BAL) as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes, and bacterial lung colonisation.MethodsWe used BAL specimens from a cohort of COPD patients and healthy controls. Bile acids were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL cell pellets and quantified using qPCR. We profiled the BAL microbiota using an amplicon sequencing approach targeting the V3-V4 region of the 16S rRNA gene.ResultsDetection of BAs in BAL was more likely at earliest clinical stages of COPD and was independent of the degree of airway obstruction. BAL specimens with BAs demonstrated higher bacterial biomass and lower diversity. Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. Detection of BAs in BAL was not associated with either inflammatory markers or clinical outcomes. We also observed different bacterial community types in BAL, which were associated with different clinical groups, levels of inflammatory markers, and the degree of airway obstruction.ConclusionDetection of BAs in BAL was associated with different parameters of airway ecology. Further studies are needed to evaluate whether BAs in BAL can be used to stratify patients and for predicting disease progression trajectories.

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    Authors: Katharina Hölscher; Niki Frantzeskaki; Marcus Collier; Stuart Connop; +26 Authors

    Abstract This paper identifies and examines the strategies and governance conditions by which ten European cities have started mainstreaming nature-based solutions (NBS) as innovative systemic solutions in urban planning. The three sets of mainstreaming strategies focus on how teams of policy entrepreneurs within the cities experimented with innovative governance processes and mechanisms to strategically develop procedures, knowledge, skills, and relationships to integrate (a) a systems’ approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised interventions, and (c) reflexivity and learning about how they interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies demonstrate how starting from NBS as a type of systemic innovation can help promote shifts in urban planning to address complex sustainability problems in cities, and how starting points and specific processes depend on a respective city’s contexts and needs.

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    https://doi.org/10.21203/rs.3....
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      https://doi.org/10.21203/rs.3....
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