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This paper seeks to understand how co-production can become embedded as a collaborative governance practice by which city governments plan, deliver and steward nature-based solutions. To these ends, the paper analyses how policy officers manifest capacities for co-production in three European cities – Genk (Belgium), Glasgow (United Kingdom), and Poznań (Poland) – while experimenting with co-production to develop and scale nature-based solutions. Co-production capacities include conditions and activities to (1) create space for co-production, (2) safeguard inclusive and legitimate co-production, and (3) link co-production processes and results to con- texts. The results demonstrate how policy officers in the three cities have mobilised and created resources, skills, institutional support and partnerships to implement diverse processes to co-produce nature-based solutions. While these conditions mark starting changes in urban governance, engaging with and embedding co-production causes tensions between the dynamic and diffuse nature of co-production and existing formal governance settings and processes. Lessons for strengthening the capacities to embed co-production as a collaborative governance practice in nature-based solutions planning, delivery, and stewardship are: (1) embedding a tailor-made approach for inclusive co-production to meaningfully engage diverse actors in place-based settings, (2) embedding open-ended co-production with long-term benefits, and (3) embedding new relations and roles to sustain co- production.

Mainstreaming nature-based solutions in cities has grown in scale and magnitude in recent times but is still considered to be the main challenge for transitioning our cities and their communities to be more climate resilient and liveable: environmentally, economically, and socially. Furthermore, taking nature-based solutions to the next level, and scaling them out to all urban contexts to achieve a greater impact, is proving to be slow and often conflicts with other transitioning initiatives such as energy generation, mobility and transport initiatives, and infilling to combat sprawl. So, the task is neither easy nor straightforward; there are many barriers to this novel transition, especially when it comes to collaborative approaches to implementing nature-based solutions with diverse urban communities and within city authorities themselves. This paper reports on a new process that is systematically co-produced and captured as a framework for planning nature-based solutions that emerged during the Connecting Nature project. The Connecting Nature Framework is a three-stage, iterative process that involves seven key activity areas for mainstreaming nature-based solutions: technical solutions, governance, financing and business models, nature-based enterprises, co-production, reflexive monitoring, and impact assessment. The tested and applied framework is designed to address and overcome barriers to the implementation of nature-based solutions in cities via a co-created, iterative, and reflective approach. The planning process guided by the proposed framework has already yielded promising results with some of the cities of the project, though further usage and its adoption by other cities is needed to explore its potential in different contexts, especially in the Global South. The paper concludes with suggestions on how this may be realised.

PUBLISHED This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding of how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver, and steward NBS. Based on these activities of the policy officers, the analysis identifies three strategies, associated stepping stones and changes in governance conditions, to mainstream NBS in governance capacities: institutionalising (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements. This paper explores the institutional mainstreaming of nature-based solutions (NBS) to advance a process-based understanding about how to strategically develop the governance capacities needed for systemic, localised and inclusive NBS. To this end, it reports how policy officers in ten European cities have started to mainstream NBS by interacting with and changing incumbent governance arrangements when experimenting with novel governance processes and mechanisms to plan, deliver and steward NBS. The analysis identifies three mainstreaming strategies to develop capacities for (a) a systems? approach to link NBS to policies, regulations, and departments across goals and sectors, (b) inclusive collaborations for localised and inclusive interventions, and (c) reflexivity and learning about how NBS interact with the (institutional, ecological, social, etc.) contexts and create impacts. The strategies illustrate institutional entrepreneurship in interacting with incumbent governance contexts, and how starting from NBS as a type of systemic innovation can promote broader shifts in urban governance arrangements.

This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested applying standard methods specifying incubation at 28 °C for 24-28h. Aggregated MIC distributions for a total of 247 isolates were determined in nine laboratories for eleven agents. Data aggregations of the disc zone for the ten agents analysed contained between 157 and 218 observations made by four to seven laboratories. Acceptable ranges for quality control (QC) reference strains were available for seven agents and the related multi-laboratory aggregated data were censored excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg/ml), gentamicin (≤4 µg/ml), oxytetracycline (≤0.25 µg/ml) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg/ml). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available or not, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole) and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to CLSI for their consideration in setting international consensus epidemiological cut-off values. International audience

In the last decade, an enormous amount of georeferenced oceanographic data, co-located with catch information, have been collected through the use of commercial fishing vessels operating in the Adriatic Sea and belonging to the fleet monitored by the AdriFOOS (Adriatic Fishery and Oceanography Observing System) infrastructure. This document describes the dataset of depth (pressure) and temperature profiles collected by means of AdriFOOS in the period 2012–2020 (available at https://doi.org/10.17882/73008, Penna et al., 2020) and briefly illustrates the structure of this facility, the oceanographic dataset validation procedures, and some of the results achieved, and it also lists some possible operational applications. This information is of the utmost importance to provide data to feed oceanographic models and advance knowledge on climate change, as well as to improve the ecosystem approach to fishery management.

The lack of internationally harmonised criteria for interpreting the data generated by standardised susceptibility testing methods presents a serious obstacle for the development of prudent use of antimicrobials in aquaculture. The data required to set epidemiological cut-off values for minimum inhibitory concentrations for antibiotic agents against Vibrio harveyi was determined using a standard microdilution method that specified the use of cation-adjusted Mueller Hinton broth and incubation at 28°C for 24 to 28 h. In total, 120 observations were made in 4 independent laboratories from 109 unique isolates. The aggregated data from these laboratories were analysed by the normalised resistance method and by ECOFFinder to calculate epidemiological cut-off values. The data for chloramphenicol, meropenem and sulfamethoxazole were not considered as suitable for analysis. The data for ampicillin indicated that this species is innately resistant to this agent. No acceptable ranges for quality control strains have been set for ceftazidime and, therefore, only provisional cut-off values could be generated for this agent. The epidemiological cut-off values were, however, calculated for the other 6 agents. These values were ≤0.5 µg ml-1 for enrofloxacin, ≤1 µg ml-1 for florfenicol, oxolinic acid and oxytetracycline, ≤4 µg ml-1 for gentamicin and ≤0.5/9.5 µg ml-1 for trimethoprim/sulfamethoxazole. Evidence is presented demonstrating that the data for these 6 antimicrobial agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for V. harveyi.

This article investigates the potential of renewable and low-carbon fuel production for the maritime shipping sector, using Sweden as a case in focus. Techno-economic modelling and socio-technical transition studies are combined to explore the conditions, opportunities and barriers to decarbonising the maritime shipping industry. A set of scenarios have been developed considering demand assumptions and potential instruments such as carbon price, energy tax, and blending mandate. The study finds that there are opportunities for decarbonising the maritime shipping industry by using renewable marine fuels such as advanced biofuels (e.g., biomethanol), electrofuels (e.g., e-methanol) and hydrogen. Sweden has tremendous resource potential for bio-based and hydrogen-based renewable liquid fuel production. In the evaluated system boundary, biomethanol presents the cheapest technology option while e-ammonia is the most expensive one. Green electricity plays an important role in the decarbonisation of the maritime sector. The results of the supply chain optimisation identify the location sites and technology in Sweden as well as the trade flows to bring the fuels to where the bunker facilities are potentially located. Biomethanol and hydrogen-based marine fuels are cost-effective at a carbon price beyond 100 €/tCO2 and 200 €/tCO2 respectively. Linking back to the socio-technical transition pathways, the study finds that some shipping companies are in the process of transitioning towards using renewable marine fuels, thereby enabling niche innovations to break through the carbon lock-in and eventually alter the socio-technical regime, while other shipping companies are more resistant. Overall, there is increasing pressure from (inter)national energy and climate policy-making to decarbonise the maritime shipping industry.