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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: González Flo, Eva; Ortiz Ruiz, Antonio; Arias, Carlos Alberto; Díez Montero, Rubén; +1 Authors

    The management and disposal of wastewater treatment sludge can be a costly and resource-intensive process. To provide a cost-effective and sustainable alternative, Sludge Treatment Wetlands (STW) have emerged as a viable solution for enhancing sludge quality through dewatering and biodegradation. In this study, the effectiveness of a full-scale STW for stabilizing and dewatering digested microalgal biomass from a domestic and agricultural wastewater treatment system was evaluated. The properties of the treated digestate in the STW were assessed after 35 weeks of operation and a resting period of 4 weeks. The dry matter content was found to be 12.8%, and the average macronutrient content was K: 3.8 mg/g DW, P: 4.9 mg/g DW, and Ca: 95 mg/g DW. The highest contents of micronutrients were for Fe: 7.8 mg/g DW and Mg: 7.6 mg/g DW, while heavy metals and pathogen contents were below the EC limits for sewage sludge reuse in agriculture. The STW was found to be a cost-effective and environmentally friendly option for treating mixed wastewater-based sludge for land application. The STW outperformed reference systems using centrifuge dewatering techniques, particularly in terms of eutrophication potential and acidification potential. However, the STW’s economic performance was slightly worse than that of the dewatering system in terms of unit production cost. This study is the first in the literature to investigate the use of STW for treating digested microalgae and its possible reuse in arable land, suggesting that STW infrastructures have great potential for the development of sustainable and eco-friendly sludge treatment technologies. By Eva Gonzalez-Flo 1 , Antonio Ortiz 2, Carlos A. Arias 3, Rubén Díez-Montero 2,4, Norbert Kohlheb 5, Ulf-Henning Schauser 6, Joan García 2 and Peder K. S. Gregersen 7. 1 GEMMA-Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Escola d’Enginyeria de Barcelona Est (EEBE), Universitat Politècnica de Catalunya-BarcelonaTech, Av. Eduard Maristany 16, Building C5.1, E-08019 Barcelona, Spain 2 GEMMA-Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Universitat Politècnica de Catalunya-BarcelonaTech, c/Jordi Girona 1-3, Building D1, E-08034 Barcelona, Spain 3 Department of Biology, Aarhus University, 8000 Aarhus, Denmark 4 GIA-Group of Environmental Engineering, Department of Water and Environmental Sciences and Technologies, Universidad de Cantabria, Avda. Los Castros s/n, 39005 Santander, Spain 5 Helmholtz-Zentrum Fuer Umveltforschung GMBH–UFZ (“UFZ”), 04318 Leipzig, Germany 6 N.A.T., Ingenieurökologisches Planungsbüro, 24340 Eckernförde, Germany 7 Center for Recirkulering v/Peder S, 6870 Gregersen Ölgod, Denmark This research was funded by the European Commission (H2020 project INCOVER, GA 689242). E. Gonzalez-Flo would like to thank the European Union-Next Generation EU, Ministry of Universities and Recovery, Transformation and Resilience Plan for her research grant [2021UPF-MS12]. A. Ortiz would like to thank the European Union Next-Generation EU, Ministry of Universities and Recovery, Transformation and Resilience Plan for his research grant [2022UPC-MSC-94120]. Peer Reviewed

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Cammareri, M; Peparaio, M; Sinesio, Fiorella; Pons, Clara; +6 Authors

    European long shelf-life (LSL) tomato landraces can be classified into two main groups: the Italian group, including ‘Piennolo’ landraces, and the Spanish group, including the ‘Penjar’ and ‘Ramellet’ landraces. In this study, a partially common set of ‘Piennolo’ and ‘Penjar-Ramellet’ varieties was grown in Italy and Spain to evaluate the effect of different agro-environmental and storage conditions on fruit quality and shelf-life. Overall, both Italian and Spanish LSL genotypes lost their peculiar phenotype in terms of physicochemical properties and shelf-life behavior when not grown in their original agro-environment. To better understand the sensory attributes that drive consumers’ preferences, all varieties were characterized using descriptive sensory analysis combined with hedonic tests. The evaluations were conducted postharvest on raw and processed products according to the country’s culinary tradition (baked in Italy; spread on bread in Spain). Sensory description by trained panels conducted on raw and processed products highlighted different sensory profiles between the two LSL tomato groups. In the ‘Piennolo’ group, a reduction in sensory diversity among the genotypes was observed in the baked products compared to the raw ones, while this trend was not observed for the ‘Penjar-Ramellet’ group. None of the varieties fully met the consumers’ preferences.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Pons, Clara; Casals Missio, Joan; Brower, Matthijs; Sacco, Adriana; +14 Authors

    European traditional tomato varieties have been selected by farmers given their consistent performance and adaptation to local growing conditions. Here we developed a multipurpose core collection, comprising 226 accessions representative of the genotypic, phenotypic, and geographical diversity present in European traditional tomatoes, to investigate the basis of their phenotypic variation, gene×environment interactions, and stability for 33 agro-morphological traits. Comparison of the traditional varieties with a modern reference panel revealed that some traditional varieties displayed excellent agronomic performance and high trait stability, as good as or better than that of their modern counterparts. We conducted genome-wide association and genome-wide environment interaction studies and detected 141 quantitative trait loci (QTLs). Out of those, 47 QTLs were associated with the phenotype mean (meanQTLs), 41 with stability (stbQTLs), and 53 QTL-by-environment interactions (QTIs). Most QTLs displayed additive gene actions, with the exception of stbQTLs, which were mostly recessive and overdominant QTLs. Both common and specific loci controlled the phenotype mean and stability variation in traditional tomato; however, a larger proportion of specific QTLs was observed, indicating that the stability gene regulatory model is the predominant one. Developmental genes tended to map close to meanQTLs, while genes involved in stress response, hormone metabolism, and signalling were found within regions affecting stability. A total of 137 marker–trait associations for phenotypic means and stability were novel, and therefore our study enhances the understanding of the genetic basis of valuable agronomic traits and opens up a new avenue for an exploitation of the allelic diversity available within European traditional tomato germplasm This work was supported by European Commission H2020 research and innovation program through TRADITOM grant agreement no. 634561, G2P-SOL, grant agreement no. 677379, and HARNESSTOM grant agreement no. 101000716. Networking activities were funded by COST Actions “EUROCAROTEN CA15136 and ‘RoxyCOST’ CA18210 ‘RoxyCOST

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Koumoundouros, George;

    This scoring sheet was developed to collect data on the larval rearing methodology which is followed by commercial finfish hatcheries. It was used by Kourkouta et al. [Sci Rep. 2022 Oct 25;12(1):17896. doi: 10.1038/s41598-022-23008-z] to examine the link between the variability in the rearing conditions, and the variability in skeletal abnormalities incidence. {"references": ["Deliverable 2.3 - E-book with quality indicators and a photo-atlas. DOI: 10.5281/zenodo.4550581", "Kourkouta C, Tsipourlianos A, Power DM, Moutou KA, Koumoundouros G. Variability of key-performance-indicators in commercial gilthead seabream hatcheries. Sci Rep. 2022 Oct 25;12(1):17896. doi: 10.1038/s41598-022-23008-z."]}

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
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    Other ORP type . 2022
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Faggion, Sara; Carnier, Paolo; Bonfatti, Valentina; Bargelloni, Luca;

    Gilthead sea bream (Sparus aurata) has been reported to be susceptible to a reassortant betanodavirus strain (RGNNV/SJNNV), posing a new threat for sea bream industry (Volpe et al. 2020) and raising the attention to selective breeding as a plausible disease prevention action. Genomic selection might be beneficial for traits, such as disease resistance, characterized by difficult, expensive and time-consuming routine individual phenotyping. Genomic models are trained firstly using a reference population of full- and half-sibs of the future breeding candidates, but, in a long-term view, the prediction of the genetic merit of future breeding candidates should be satisfactory even when the reference population consists of distant relatives of the animals to be predicted. In this sense, the genomic predictive accuracy provided by random k-fold cross-validations might be unrealistic. In this study, we assessed the accuracy of a genomic prediction model for VNN symptomatology pseudo-phenotypes (estimated breeding values, EBV) in gilthead sea bream in three different validation settings: 1) a random cross-validation; 2) a cross-validation based on genomic clustering; 3) a leave-one-family-out (LOFO) validation focused on the parents of the fish subjected to the VNN challenge test.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://doi.org/10.5...arrow_drop_down
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    https://doi.org/10.5281/zenodo...
    Other ORP type . 2022
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://doi.org/10.5...arrow_drop_down
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      https://doi.org/10.5281/zenodo...
      Other ORP type . 2022
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Ciulli, Sara; Errani, Francesca; Riera-Ferrer, Enrique; Caffara, Monica; +4 Authors

    Viral nervous necrosis (VNN) otherwise known as viral encephalopathy and retinopathy (VER), is the most threatening infectious disease in Mediterranean aquaculture. VNN is caused by the nervous necrosis virus (NNV) a bisegmented ssRNA+ virus included in the genus Betanodavirus, family Nodaviridae. NNV genome consists of two molecules named RNA1 and RNA2, which encodes for the RNA-dependent RNA polymerase and the coat protein, respectively. Four genotypes of NNV have been so far described: RGNNV, SJNNV, BFNNV, TPNNV (Sahul Hameed et al., 2019). Furthermore, reassortant strains have emerged from the reassortment between the RGNNV and the SJNNV genotypes and named RGNNV/SJNNV and SJNNV/RGNNV according to the RNA1 and RNA2 origin. Currently, several NNV strains are co-circulating in the Mediterranean Basin with a high prevalence of the RGNNV genotype and the RGNNV/SJNNV reassortant strain and a more limited diffusion of the SJNNV genotype and the SJNNV/RGNNV reassortant (Bandin and Souto, 2020; Volpe et al., 2020). So far, identification of NNV genotype and reassortant strains is based on amplification, sequencing and phylogenetic analysis of both viral genome RNA molecules requiring time and expertise. As viral identification is an essential information for the management of the disease in the field (Toffan et al., 2021), the development of an easy and affordable method to genotype betanodaviruses was developed in the framework of the H2020 Peformfish Project, an industry-driven project targeting industry defined priorities.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://doi.org/10.5...arrow_drop_down
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    https://doi.org/10.5281/zenodo...
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      https://doi.org/10.5281/zenodo...
      Other ORP type . 2022
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Constenla, M; Fioravanti, ML; Ciulli, S; Gustinelli, A; +3 Authors

    Prevention is one of the most relevant aspects to provide efficient tools for improving health management in aquaculture. However, establishing useful guidelines for this purpose is not an easy task since the development of diseases in fish are usually multifactorial. The combination and impact of different factors, such as the characteristics of the infectious agents and fish host, environmental conditions, geographical parameters, and farming systems amongst others represents the main elements that determine the risk or likelihood to be impacted by a certain disease. The knowledge of these factors allows to establish biosecurity and prophylactic measures to reduce the risks of emergence of pathogens and the susceptibility to infectious diseases. Thus, the aim of this study is to assess the relevance of these factors in order to identify the main risk factors for the most relevant diseases in gilthead seabream and European seabass in Europe

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    https://doi.org/10.5281/zenodo...
    Other ORP type . 2022
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      https://doi.org/10.5281/zenodo...
      Other ORP type . 2022
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  • Authors: Klaedtke, Stéphanie; Rey, Frédéric; Groot, Steven;

    Seed germination and seedling establishment are the main factors for a successful start of a crop, determining crop health, weed suppression and overall crop performance. They are critical, but sensitive phases in crop production. High seed vigour and appropriate seed microbiota significantly contribute to the resilience of seedlings. This opinion paper is based on a review of relevant literature, two case studies with wheat and carrot and a subsequent stakeholder consultation in the framework of the European project LIVESEED. It presents a seed health strategy for organic farming systems that accounts for dynamic processes behind seed and plant health, taking advantage of the contributions of seed vigour and seed microbiota. Seed and plant health are understood as a continuum. Benefits of seed vigour and microbiota are showcased. Recommendations are given for organic seed production, seed processing and storage, as well as organic plant breeding and future research. In conclusion, organic farming systems, in particular, have the opportunity, both to develop this perspective on seed and plant health and to benefit from it. Adopting this perspective may require a shift in how crop plants and their seeds are cared for, as well as adapted regulatory frameworks.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Rezaei, Mahdi; Azarmi, Mohsen; Mohammad Pour Mir, Farzam;

    Computer Vision has played a major role in Intelligent Transportation Systems (ITS) and traffic surveillance. Along with the rapidly growing automated vehicles and crowded cities, the automated and advanced traffic management systems (ATMS) using video surveillance infrastructures have been evolved by the implementation of Deep Neural Networks. In this research, we provide a practical platform for real-time traffic monitoring, including 3D vehicle/pedestrian detection, speed detection, trajectory estimation, congestion detection, as well as monitoring of the interaction of vehicles and pedestrians, all using a single CCTV traffic camera. We adapt a custom YOLOv5 deep neural network model for vehicle/pedestrian detection and an enhanced SORT tracking algorithm. For the first time, a hybrid satellite-ground based inverse perspective mapping (SG-IPM) method for camera auto-calibration is also developed which leads to an accurate 3D object detection and visualisation. We also develop a hierarchical traffic modelling solution based on short- and long-term temporal video data streams to understand the traffic flow, bottlenecks, and risky spots for vulnerable road users. Several experiments on real-world scenarios and comparisons with state-of-the-art are conducted using various traffic monitoring datasets, including MIO-TCD, UA-DETRAC and GRAM-RTM collected from highways, intersections, and urban areas under different lighting and weather conditions.Computer Vision has played a major role in Intelligent Transportation Systems (ITS) and traffic surveillance. Along with the rapidly growing automated vehicles and crowded cities, the automated and advanced traffic management systems (ATMS) using video surveillance infrastructures have been evolved by the implementation of Deep Neural Networks. In this research, we provide a practical platform for real-time traffic monitoring, including 3D vehicle/pedestrian detection, speed detection, trajectory estimation, congestion detection, as well as monitoring the interaction of vehicles and pedestrians, all using a single CCTV traffic camera. We adapt a custom YOLOv5 deep neural network model for vehicle/pedestrian detection and an enhanced SORT tracking algorithm. For the first time, a hybrid satellite-ground based inverse perspective mapping (SG-IPM) method for camera auto-calibration is also developed which leads to an accurate 3D object detection and visualisation. We also develop a hierarchical traffic modelling solution based on short- and long-term temporal video data streams to understand the traffic flow, bottlenecks, and risky spots for vulnerable road users. Several experiments on real-world scenarios and comparisons with state-of-the-art are conducted using various traffic monitoring datasets, including MIO-TCD, UA-DETRAC and GRAM-RTM collected from highways, intersections, and urban areas under different lighting and weather conditions.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ https://doi.org/10.5...arrow_drop_down
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    Authors: Gustinelli, A.; Caffara, M.; Magri, A.; Pierantozzi, M.; +1 Authors

    Enteromyxum leei is an enteric Myxozoan parasite causing the so-called “razor blade syndrome” in gilthead sea bream (Sparus aurata), a chronic condition characterized by a progressive weight loss up to emaciation in adult fish. Currently no treatments are found to be effective against this parasite, but a recent experimental study showed promising results using a functional feed for mitigating enteromyxosis (Palenzuela et al., 2020. Dis. Aquat. Org., 138, 111–120). During a field trial aimed to confirm the efficacy of this functional feed in an Italian sea bream facility where enteromyxosis was already known to be present, different methods for the detection of E. leei were compared to establish the more reliable diagnostic test also in relation to a non-lethal approach.

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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: González Flo, Eva; Ortiz Ruiz, Antonio; Arias, Carlos Alberto; Díez Montero, Rubén; +1 Authors

    The management and disposal of wastewater treatment sludge can be a costly and resource-intensive process. To provide a cost-effective and sustainable alternative, Sludge Treatment Wetlands (STW) have emerged as a viable solution for enhancing sludge quality through dewatering and biodegradation. In this study, the effectiveness of a full-scale STW for stabilizing and dewatering digested microalgal biomass from a domestic and agricultural wastewater treatment system was evaluated. The properties of the treated digestate in the STW were assessed after 35 weeks of operation and a resting period of 4 weeks. The dry matter content was found to be 12.8%, and the average macronutrient content was K: 3.8 mg/g DW, P: 4.9 mg/g DW, and Ca: 95 mg/g DW. The highest contents of micronutrients were for Fe: 7.8 mg/g DW and Mg: 7.6 mg/g DW, while heavy metals and pathogen contents were below the EC limits for sewage sludge reuse in agriculture. The STW was found to be a cost-effective and environmentally friendly option for treating mixed wastewater-based sludge for land application. The STW outperformed reference systems using centrifuge dewatering techniques, particularly in terms of eutrophication potential and acidification potential. However, the STW’s economic performance was slightly worse than that of the dewatering system in terms of unit production cost. This study is the first in the literature to investigate the use of STW for treating digested microalgae and its possible reuse in arable land, suggesting that STW infrastructures have great potential for the development of sustainable and eco-friendly sludge treatment technologies. By Eva Gonzalez-Flo 1 , Antonio Ortiz 2, Carlos A. Arias 3, Rubén Díez-Montero 2,4, Norbert Kohlheb 5, Ulf-Henning Schauser 6, Joan García 2 and Peder K. S. Gregersen 7. 1 GEMMA-Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Escola d’Enginyeria de Barcelona Est (EEBE), Universitat Politècnica de Catalunya-BarcelonaTech, Av. Eduard Maristany 16, Building C5.1, E-08019 Barcelona, Spain 2 GEMMA-Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Universitat Politècnica de Catalunya-BarcelonaTech, c/Jordi Girona 1-3, Building D1, E-08034 Barcelona, Spain 3 Department of Biology, Aarhus University, 8000 Aarhus, Denmark 4 GIA-Group of Environmental Engineering, Department of Water and Environmental Sciences and Technologies, Universidad de Cantabria, Avda. Los Castros s/n, 39005 Santander, Spain 5 Helmholtz-Zentrum Fuer Umveltforschung GMBH–UFZ (“UFZ”), 04318 Leipzig, Germany 6 N.A.T., Ingenieurökologisches Planungsbüro, 24340 Eckernförde, Germany 7 Center for Recirkulering v/Peder S, 6870 Gregersen Ölgod, Denmark This research was funded by the European Commission (H2020 project INCOVER, GA 689242). E. Gonzalez-Flo would like to thank the European Union-Next Generation EU, Ministry of Universities and Recovery, Transformation and Resilience Plan for her research grant [2021UPF-MS12]. A. Ortiz would like to thank the European Union Next-Generation EU, Ministry of Universities and Recovery, Transformation and Resilience Plan for his research grant [2022UPC-MSC-94120]. Peer Reviewed

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Cammareri, M; Peparaio, M; Sinesio, Fiorella; Pons, Clara; +6 Authors

    European long shelf-life (LSL) tomato landraces can be classified into two main groups: the Italian group, including ‘Piennolo’ landraces, and the Spanish group, including the ‘Penjar’ and ‘Ramellet’ landraces. In this study, a partially common set of ‘Piennolo’ and ‘Penjar-Ramellet’ varieties was grown in Italy and Spain to evaluate the effect of different agro-environmental and storage conditions on fruit quality and shelf-life. Overall, both Italian and Spanish LSL genotypes lost their peculiar phenotype in terms of physicochemical properties and shelf-life behavior when not grown in their original agro-environment. To better understand the sensory attributes that drive consumers’ preferences, all varieties were characterized using descriptive sensory analysis combined with hedonic tests. The evaluations were conducted postharvest on raw and processed products according to the country’s culinary tradition (baked in Italy; spread on bread in Spain). Sensory description by trained panels conducted on raw and processed products highlighted different sensory profiles between the two LSL tomato groups. In the ‘Piennolo’ group, a reduction in sensory diversity among the genotypes was observed in the baked products compared to the raw ones, while this trend was not observed for the ‘Penjar-Ramellet’ group. None of the varieties fully met the consumers’ preferences.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Pons, Clara; Casals Missio, Joan; Brower, Matthijs; Sacco, Adriana; +14 Authors

    European traditional tomato varieties have been selected by farmers given their consistent performance and adaptation to local growing conditions. Here we developed a multipurpose core collection, comprising 226 accessions representative of the genotypic, phenotypic, and geographical diversity present in European traditional tomatoes, to investigate the basis of their phenotypic variation, gene×environment interactions, and stability for 33 agro-morphological traits. Comparison of the traditional varieties with a modern reference panel revealed that some traditional varieties displayed excellent agronomic performance and high trait stability, as good as or better than that of their modern counterparts. We conducted genome-wide association and genome-wide environment interaction studies and detected 141 quantitative trait loci (QTLs). Out of those, 47 QTLs were associated with the phenotype mean (meanQTLs), 41 with stability (stbQTLs), and 53 QTL-by-environment interactions (QTIs). Most QTLs displayed additive gene actions, with the exception of stbQTLs, which were mostly recessive and overdominant QTLs. Both common and specific loci controlled the phenotype mean and stability variation in traditional tomato; however, a larger proportion of specific QTLs was observed, indicating that the stability gene regulatory model is the predominant one. Developmental genes tended to map close to meanQTLs, while genes involved in stress response, hormone metabolism, and signalling were found within regions affecting stability. A total of 137 marker–trait associations for phenotypic means and stability were novel, and therefore our study enhances the understanding of the genetic basis of valuable agronomic traits and opens up a new avenue for an exploitation of the allelic diversity available within European traditional tomato germplasm This work was supported by European Commission H2020 research and innovation program through TRADITOM grant agreement no. 634561, G2P-SOL, grant agreement no. 677379, and HARNESSTOM grant agreement no. 101000716. Networking activities were funded by COST Actions “EUROCAROTEN CA15136 and ‘RoxyCOST’ CA18210 ‘RoxyCOST

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Recolector de Cienci...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Koumoundouros, George;

    This scoring sheet was developed to collect data on the larval rearing methodology which is followed by commercial finfish hatcheries. It was used by Kourkouta et al. [Sci Rep. 2022 Oct 25;12(1):17896. doi: 10.1038/s41598-022-23008-z] to examine the link between the variability in the rearing conditions, and the variability in skeletal abnormalities incidence. {"references": ["Deliverable 2.3 - E-book with quality indicators and a photo-atlas. DOI: 10.5281/zenodo.4550581", "Kourkouta C, Tsipourlianos A, Power DM, Moutou KA, Koumoundouros G. Variability of key-performance-indicators in commercial gilthead seabream hatcheries. Sci Rep. 2022 Oct 25;12(1):17896. doi: 10.1038/s41598-022-23008-z."]}

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ ZENODOarrow_drop_down
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Faggion, Sara; Carnier, Paolo; Bonfatti, Valentina; Bargelloni, Luca;

    Gilthead sea bream (Sparus aurata) has been reported to be susceptible to a reassortant betanodavirus strain (RGNNV/SJNNV), posing a new threat for sea bream industry (Volpe et al. 2020) and raising the attention to selective breeding as a plausible disease prevention action. Genomic selection might be beneficial for traits, such as disease resistance, characterized by difficult, expensive and time-consuming routine individual phenotyping. Genomic models are trained firstly using a reference population of full- and half-sibs of the future breeding candidates, but, in a long-term view, the prediction of the genetic merit of future breeding candidates should be satisfactory even when the reference population consists of distant relatives of the animals to be predicted. In this sense, the genomic predictive accuracy provided by random k-fold cross-validations might be unrealistic. In this study, we assessed the accuracy of a genomic prediction model for VNN symptomatology pseudo-phenotypes (estimated breeding values, EBV) in gilthead sea bream in three different validation settings: 1) a random cross-validation; 2) a cross-validation based on genomic clustering; 3) a leave-one-family-out (LOFO) validation focused on the parents of the fish subjected to the VNN challenge test.

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    Authors: Ciulli, Sara; Errani, Francesca; Riera-Ferrer, Enrique; Caffara, Monica; +4 Authors

    Viral nervous necrosis (VNN) otherwise known as viral encephalopathy and retinopathy (VER), is the most threatening infectious disease in Mediterranean aquaculture. VNN is caused by the nervous necrosis virus (NNV) a bisegmented ssRNA+ virus included in the genus Betanodavirus, family Nodaviridae. NNV genome consists of two molecules named RNA1 and RNA2, which encodes for the RNA-dependent RNA polymerase and the coat protein, respectively. Four genotypes of NNV have been so far described: RGNNV, SJNNV, BFNNV, TPNNV (Sahul Hameed et al., 2019). Furthermore, reassortant strains have emerged from the reassortment between the RGNNV and the SJNNV genotypes and named RGNNV/SJNNV and SJNNV/RGNNV according to the RNA1 and RNA2 origin. Currently, several NNV strains are co-circulating in the Mediterranean Basin with a high prevalence of the RGNNV genotype and the RGNNV/SJNNV reassortant strain and a more limited diffusion of the SJNNV genotype and the SJNNV/RGNNV reassortant (Bandin and Souto, 2020; Volpe et al., 2020). So far, identification of NNV genotype and reassortant strains is based on amplification, sequencing and phylogenetic analysis of both viral genome RNA molecules requiring time and expertise. As viral identification is an essential information for the management of the disease in the field (Toffan et al., 2021), the development of an easy and affordable method to genotype betanodaviruses was developed in the framework of the H2020 Peformfish Project, an industry-driven project targeting industry defined priorities.

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    Authors: Constenla, M; Fioravanti, ML; Ciulli, S; Gustinelli, A; +3 Authors

    Prevention is one of the most relevant aspects to provide efficient tools for improving health management in aquaculture. However, establishing useful guidelines for this purpose is not an easy task since the development of diseases in fish are usually multifactorial. The combination and impact of different factors, such as the characteristics of the infectious agents and fish host, environmental conditions, geographical parameters, and farming systems amongst others represents the main elements that determine the risk or likelihood to be impacted by a certain disease. The knowledge of these factors allows to establish biosecurity and prophylactic measures to reduce the risks of emergence of pathogens and the susceptibility to infectious diseases. Thus, the aim of this study is to assess the relevance of these factors in order to identify the main risk factors for the most relevant diseases in gilthead seabream and European seabass in Europe

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  • Authors: Klaedtke, Stéphanie; Rey, Frédéric; Groot, Steven;

    Seed germination and seedling establishment are the main factors for a successful start of a crop, determining crop health, weed suppression and overall crop performance. They are critical, but sensitive phases in crop production. High seed vigour and appropriate seed microbiota significantly contribute to the resilience of seedlings. This opinion paper is based on a review of relevant literature, two case studies with wheat and carrot and a subsequent stakeholder consultation in the framework of the European project LIVESEED. It presents a seed health strategy for organic farming systems that accounts for dynamic processes behind seed and plant health, taking advantage of the contributions of seed vigour and seed microbiota. Seed and plant health are understood as a continuum. Benefits of seed vigour and microbiota are showcased. Recommendations are given for organic seed production, seed processing and storage, as well as organic plant breeding and future research. In conclusion, organic farming systems, in particular, have the opportunity, both to develop this perspective on seed and plant health and to benefit from it. Adopting this perspective may require a shift in how crop plants and their seeds are cared for, as well as adapted regulatory frameworks.

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    Authors: Rezaei, Mahdi; Azarmi, Mohsen; Mohammad Pour Mir, Farzam;

    Computer Vision has played a major role in Intelligent Transportation Systems (ITS) and traffic surveillance. Along with the rapidly growing automated vehicles and crowded cities, the automated and advanced traffic management systems (ATMS) using video surveillance infrastructures have been evolved by the implementation of Deep Neural Networks. In this research, we provide a practical platform for real-time traffic monitoring, including 3D vehicle/pedestrian detection, speed detection, trajectory estimation, congestion detection, as well as monitoring of the interaction of vehicles and pedestrians, all using a single CCTV traffic camera. We adapt a custom YOLOv5 deep neural network model for vehicle/pedestrian detection and an enhanced SORT tracking algorithm. For the first time, a hybrid satellite-ground based inverse perspective mapping (SG-IPM) method for camera auto-calibration is also developed which leads to an accurate 3D object detection and visualisation. We also develop a hierarchical traffic modelling solution based on short- and long-term temporal video data streams to understand the traffic flow, bottlenecks, and risky spots for vulnerable road users. Several experiments on real-world scenarios and comparisons with state-of-the-art are conducted using various traffic monitoring datasets, including MIO-TCD, UA-DETRAC and GRAM-RTM collected from highways, intersections, and urban areas under different lighting and weather conditions.Computer Vision has played a major role in Intelligent Transportation Systems (ITS) and traffic surveillance. Along with the rapidly growing automated vehicles and crowded cities, the automated and advanced traffic management systems (ATMS) using video surveillance infrastructures have been evolved by the implementation of Deep Neural Networks. In this research, we provide a practical platform for real-time traffic monitoring, including 3D vehicle/pedestrian detection, speed detection, trajectory estimation, congestion detection, as well as monitoring the interaction of vehicles and pedestrians, all using a single CCTV traffic camera. We adapt a custom YOLOv5 deep neural network model for vehicle/pedestrian detection and an enhanced SORT tracking algorithm. For the first time, a hybrid satellite-ground based inverse perspective mapping (SG-IPM) method for camera auto-calibration is also developed which leads to an accurate 3D object detection and visualisation. We also develop a hierarchical traffic modelling solution based on short- and long-term temporal video data streams to understand the traffic flow, bottlenecks, and risky spots for vulnerable road users. Several experiments on real-world scenarios and comparisons with state-of-the-art are conducted using various traffic monitoring datasets, including MIO-TCD, UA-DETRAC and GRAM-RTM collected from highways, intersections, and urban areas under different lighting and weather conditions.

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    Authors: Gustinelli, A.; Caffara, M.; Magri, A.; Pierantozzi, M.; +1 Authors

    Enteromyxum leei is an enteric Myxozoan parasite causing the so-called “razor blade syndrome” in gilthead sea bream (Sparus aurata), a chronic condition characterized by a progressive weight loss up to emaciation in adult fish. Currently no treatments are found to be effective against this parasite, but a recent experimental study showed promising results using a functional feed for mitigating enteromyxosis (Palenzuela et al., 2020. Dis. Aquat. Org., 138, 111–120). During a field trial aimed to confirm the efficacy of this functional feed in an Italian sea bream facility where enteromyxosis was already known to be present, different methods for the detection of E. leei were compared to establish the more reliable diagnostic test also in relation to a non-lethal approach.

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